P2Y12 Receptor Blocking Peptide (#BLP-PR012) is the original antigen used for immunization during Anti-P2Y12 Receptor Antibody (#APR-012) generation. The blocking peptide binds and ‘blocks’ Anti-P2Y12 Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes (1 and 2) and human platelets (3 and 4):
- Western blot analysis of human platelets:
- Expression of P2RY12 in mouse brainImmunohistochemical staining of mouse brain sections using Anti-P2Y12 Receptor Antibody (#APR-012), (red). The region shown in this picture is the entorhinal cortex in mouse brain. The P2Y12 positive fibers (arrows) run from the corpus callosum (triangles) into the deep layers of the entorhinal cortex. Sytox green is used as the counterstain.
- (C)KTTRPFKTSNPKNLLGAK, corresponding to amino acid residues 125-142 of human P2RY12 (Accession Q9H244).