Anti-Endothelin Receptor A Antibody

EDNRA, ETA, ET-AR, ETRA, ET1-Specific type endothelin receptor, Endothelin-1 receptor
    Cat #: AER-001
    Alternative Name EDNRA, ETA, ET-AR, ETRA, ET1-Specific type endothelin receptor, Endothelin-1 receptor
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    May also work in: h*
    Immunogen
    • Peptide (C)NHNTERSSHKDSMN, corresponding to amino acid residues 413-426 of rat ET-A (Accession P26684). Intracellular, C-terminus.
    • Anti-Endothelin Receptor A Antibody
    Accession (Uniprot) Number P26684
    Gene ID 24326
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human, swine, bovine - 13/14 amino acid residues identical; chicken - 12/14 amino acid residues identical.
    RRID AB_2039838.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.9 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, wb
    May also work in: ifc*, ip*
    Western blot
    • Anti-Endothelin Receptor A Antibody
      Western blot analysis of rat brain membranes:
      1. Anti-Endothelin Receptor A Antibody (#AER-001), (1:200).
      2. Anti-Endothelin Receptor A Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    • Anti-Endothelin Receptor A Antibody
      Expression of ET-A in rat lung
      Immunohistochemical staining of paraffin embedded section of rat lung using Anti-Endothelin Receptor A Antibody (#AER-001), (1:100). ET-A is expressed both in respiratory epithelium (black arrows) and in respiratory smooth muscle (red arrows). Hematoxilin is used as the counterstain.
    • Anti-Endothelin Receptor A Antibody
      Expression of ET-A in rat cerebellum
      Immunohistochemical staining of rat cerebellum using Anti-Endothelin Receptor A Antibody (#AER-001) (A), and Anti-Endothelin Receptor A  Antibody preincubated with the control peptide antigen (B). Cresyl violet is used as the counterstain.
    • Mouse retinas (Bramall, A.N. et al. (2013) PLoS ONE 8, e58023.)
    Immunocytochemistry
    • Mouse isolated juxtaglomerular (JG) primary cells (1:100) (Ortiz-Capisano, M.C. (2014) Physiol. Rep. 2, e12240.).
    References
    1. Davenport, A.P. (2002) Pharmacol. Rev. 54, 219.
    2. Boivin, B. et al. (2003) J. Biol. Chem. 278, 29153.
    3. Grant, K. et al. (2003) Br. J. Cancer 88, 163.
    4. Asham, E. et al. (2001) Br. J. Cancer 85, 1759.
    5. Kopetz, E.S. et al. (2002) Invest. New Drugs 20, 173.
    6. Rosano, L. et al. (2003) Am. J. Pathol. 163, 753.
    7. Rosano, L. et al. (2003) Cancer Res. 63, 2447.
    8. Wulfing, P. et al. (2003) Clin. Cancer Res. 9, 4125.
    9. Nelson, J.B. (2003) J. Urol. 170, S65.
    Scientific background

    The endothelin system is comprised of three active peptides, ET-1, 2, and 3, which are considered to be very powerful vasoconstrictive substances. In humans, endothelins mediate their actions via two specific G-Protein Coupled Receptors, ETAR and ETBR. Both ETAR and ETBR are present in heart and in human myocardium at similar levels.1,2

    The endothelin receptors differ in their ligand specificity. While ETAR has varying affinities for the endothelin isoforms (ET-1 >ET-2>ET-3), ETBR shows no selective affinity.2,3 Subsequent studies have demonstrated the presence of endothelins in vascular as well as in non-vascular cells and tissues, having multiple biological activities.

    Currently, there is increasing evidence that ET-1 may modulate mitogenesis, apoptosis, angiogenesis tumor invasion and the development of metastases.3

    Overexpression of ET-1 and ETAR was reported in different malignancies including prostate cancer human Kaposi’s sarcoma, ovarian and breast carcinomas.4-9

    In breast carcinomas overexpression of ET-1 and ETA receptors correlated with parameters that characterize aggressive types of breast cancer15 suggesting that analysis of ETAR expression might be used as a diagnostic marker for evaluating the progression of the disease and effectiveness of treatment. These and other findings have made ET receptors, and especially ETAR, promising therapeutic targets for pharmacological intervention.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:

    Anti-Endothelin Receptor A Antibody
    Expression of Endothelin Receptor A in mouse JG cells.Immunocytochemical staining of mouse isolated juxtaglomerular (JG) cells using Anti-Endothelin Receptor A Antibody (#AER-001), (red). ET-A staining co-localizes with renin, a kidney cell marker, (green).Adapted from Ortiz-Capisano, M.C. (2014) Physiol. Rep. 2, e12240. with permission of the American Physiological Society and The Physiological Society.

    Last update: 24/01/2020

    Anti-Endothelin Receptor A Antibody (#AER-001) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize ET-AR from rat, mouse, and human samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Western blot citations
    1. Rat arterial tissue lysate.
      Amor, S. et al. (2017) Exp. Gerontol. 88, 32.
    2. Rat paraventricular nucleus (PVN) lysate.
      Subramanian, M. et al. (2017) Sci. Rep. 7, 139.
    3. Swine femoral artery smooth muscle lysate (1:200).
      Bender, S.B. et al. (2014) J. Physiol. 592, 1757.
    4. Rat spinal cord lysate (1:150).
      Forner, S. et al. (2016) Neurosci. Lett. 617, 14.
    5. Rat kidney lysate.
      Ogura, Y. et al. (2014) Life Sci. 118, 347.
    6. Rat heart lysate.
      Seki, Y. et al. (2014) Life Sci. 118, 357.
    Immunohistochemistry citations
    1. Rat epididymal fat sections.
      Briançon-Marjollet, A. et al. (2016) J. Physiol. 594, 1727.
    2. Rat posterior cerebral artery sections.
      Durgan, D.J. et al. (2015) J. Cereb. Blood Flow Metab. 35, 402.
    3. Mouse kidney sections.
      Ortiz-Capisano, M.C. (2014) Physiol. Rep. 2, e12240.
    4. Rat penis section (1:100).
      Sanchez, A. et al. (2014) Br. J. Pharmacol. 171, 5682.
    5. Mouse retina sections.
      Bramall, A.N. et al. (2013) PLoS ONE 8, e58023.
    Immunocytochemistry citations
    1. Mouse isolated juxtaglomerular (JG) primary cells (1:100).
      Ortiz-Capisano, M.C. (2014) Physiol. Rep. 2, e12240.
    Shipping and Ordering information