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SB 203580

A MAPK Homologue-p38 Inhibitor

Cat #: S-370
Lyophilized Powder yes
  • Bioassay Tested
    • Source Synthetic
    MW: 377.44
    Purity: >99%
    Effective concentration 10-100 µM.
    Structure
    Chemical name 4-[5-(4-Fluorophenyl)-2-[4-(methylsulfonyl)phenyl]- 1H-imidazol-4-yl]pyridine.
    Molecular formula C21H16FN3OS.
    CAS No.: 152121-47-6
    Activity SB 203580 is a specific inhibitor for the p38 MAP kinase homologues1-6.
    References-Activity
    1. Lee, J.C. et al. (1994) Nature 372, 739.
    2. Cuenda, A. et al. (1995) FEBS Lett. 364, 229.
    3. Whitmarsh, A.J. et al. (1997) Mol. Cell. Biol. 17, 2360.
    4. Clerk, A. et al. (1998) FEBS Lett. 426, 93.
    5. Hunt, A.E. et al. (1999) J. Biol. Chem. 274, 7591.
    6. Ward, S.G. et al. (1997) Biochem. Soc. Trans. 2, 304S.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to two weeks at 4°C or six months at -20°C.
    Protect from light.
    Our bioassay
    • Alomone Labs SB 203580 inhibits the phosphorylation of Elk1 and Atf2 via p38 in 3T3-L1 cells.
      Alomone Labs SB 203580 inhibits the phosphorylation of Elk1 and Atf2 via p38 in 3T3-L1 cells.
      Cells were grown to 70% confluence and serum starved for 3 h. The cells were then incubated for 2 h with various concentrations of SB 203580 (#S-370) and stimulated with 5 µM Anisomycin (#A-520). Cell proteins were resolved by SDS-PAGE and probed with anti-phospho-p38 MAPK, antip38 MAPK, anti-phospho-Elk1 and anti-phospho-Atf2 as indicated. The phosphorylation of Elk1 and Atf2 decreased in a dose-dependent manner.
    References - Scientific background
    1. Lee, J.C. et al. (1994) Nature 372, 739.
    2. Cuenda, A. et al. (1995) FEBS Lett. 364, 229.
    3. Whitmarsh, A.J. et al. (1997) Mol. Cell. Biol. 17, 2360.
    4. Clerk, A. et al. (1998) FEBS Lett. 426, 93.
    5. Hunt, A.E. et al. (1999) J. Biol. Chem. 274, 7591.
    6. Ward, S.G. et al. (1997) Biochem. Soc. Trans. 2, 304S.
    7. Lali F.V. et al. (2000) J. Biol. Chem. 275, 7395.
    8. Martin, M. et al. (2003) Infect. Immun. 71, 2498.
    9. Darveau, R.P. et al. (2002) Infect. Immun. 70, 1867.
    Scientific background SB203580 is highly selective and cell permeable pyridinyl imidazole that inhibits the activity of MAP kinase homologs - p38 (stress-activated protein kinase II/HOG1) and p54 (stress-activated protein kinase I/c-Jun N-terminal kinase) with IC50 values of 50 and 500 nM for p38/SAPK2a and p38/SAPK2b respectively.1-6 SB203580 also inhibits the phosphorylation and activation of protein kinase B (PKB, also known as Akt) by inhibiting the PKB kinase, phosphoinositide-dependent protein kinase 1 (IC50 3-5 µM).7-9
    Lyophilized Powder
    Last update: 11/04/2021

    SB 20358 (#S-370) is a highly pure, synthetic, and biologically active compound.

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