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- Alomone Labs Staurosporine inhibits MARCKS phosphorylation via PKC in C6 glioma cells.Cells were grown to 70% confluence and then serum starved for 18 h. The cells were preincubated for 30 min with different concentrations of Staurosporine (#S-350) and stimulated with 2 µM PMA (#P-800) for 30 min. Cell proteins were resolved by SDS-PAGE and probed with antiphospho (ser)-MARCKS (myristoylated alanin-rich protein kinase C substrate).
- Alomone Labs Staurosporine induces apoptosis in Jurkat cells.Cells were grown to 70% confluency then 2 µM Staurosporine (#S-350) or vehicle were added for 3 or 6 hours. At the end of the incubation period, cell proteins were resolved by SDS-PAGE and probed with Caspase 3 and cleaved Caspase 3 specific antibodies.
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Staurosporine is a member of the K252a family of fungal alkaloids produce by Streptomyces staurospores.1,2 It is one of the most potent, cell permeable protein kinase inhibitors, and it is often used to study the involvement of protein kinases in signal transduction pathways (IC50 0.7-20 nM).3-10
In pheochromocytoma PC12 cells, neuroblastoma and brain primary culture Staurosporine is a functional neurotropins agonist promoting neurite outgrowth (EC50 = 50 nM).11-12 At concentrations higher then 0.5 mM, Staurosporine generates apoptosis in several different cell types or induces G1 and G2 phase arrest in normal cells.13-16
Staurosporine (#S-350) is a highly pure, natural, and biologically active compound.