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Tetanus Toxin

Tentoxylysin

An Inhibitor of Neurotransmitter Release

Cat #: T-150
Alternative Name Tentoxylysin
Lyophilized Powder yes
  • Bioassay Tested
  • Origin Natural protein isolated from Clostridium tetani.
    MW: 150 kDa
    Purity: >97% (HPLC)
    Effective concentration 1-100 nM.
    Modifications Disulfide bonds between Cys439-Cys467 and Cys1077-Cys1093.
    CAS No.: 676570-37-9
    Activity Tetanus Toxin inhibits neurotransmitter release by cleaving synaptobrevin-2 in a zinc-dependent manner1,2.
    References-Activity
    1. Schiavo, G. et al. (1992) EMBO J11, 3577.
    2. Schiavo, G. et al. (1992) Nature 359, 832.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any conventional buffer containing 0.01 KH2PO4, 0.1% gelatin and/or 0.1% bovine serum albumin (BSA). It is recommended to use gelatin or 0.01% BSA coated/treated glassware. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to one week at 4°C. Do not freeze.
    Our bioassay
    • The activity of this lot was confirmed by several assays: upon injection to Balb C mice, a value of about 4x107 MLD (median lethal doses) per mg protein has been obtained and typical Tetanus syndromes have been observed. The toxin also efficiently inhibited Ca2+-induced catecholamine release from permeabilized chromaffinergic neurons7.
    Note Tetanus Toxin is extremely toxic and hazardous. Each user should be immunized according to health authority instructions (2-3 months upon active immunization, a protective titer is generally reached in humans allowing protection and safe laboratory handling). Tetanus Toxin preparation is strictly for laboratory research and not for any clinical human purpose. Do not use syringe or sharp objects to remove the toxin from the vial. After opening, use disposable materials. Tetanus Toxin handling requires the use of gloves and decontamination using 0.01% formaldehyde before discarding.
    References - Scientific background
    1. Matsuda, M. and Yoneda, M. (1975) Infect. Immun. 12, 1147.
    2. Laemmli, V.K. (1970) Nature 227, 680.
    3. T. M. Cooket al. (2001) Br. J. Anaesth87, 477.
    4. Niemann, H. (1991) in A Sourcebook of Bacterial Protein Toxins (Alouf J. E., Freer J. H. eds), Academic Press, London 303.
    5. Lalli, G. et al. (2003) J. Cell Sci. 116, 4639.
    6. Schiavo, G. et al. (1992). Nature 359, 832.
    7. Bittner, M.A. and Holz, R.W. (1988) Neurochem. 51, 451.
    Scientific background

    Tetanus toxin is a 150 kD protein produced by the gram positive bacteria Clostridium tetani under anaerobic condition1. Tetanus toxin is a hetrodimer of A-chain (50 kD) and B-chain (100 kD) linked by one disulfide bond2,3. The A-chain has a zinc endopeptidase activity while the B-chain has a role in toxin anchoring to neuronal membranes4.

    Tetanus toxin spreads in the body through the lymphatic and vascular systems to the neuromuscular junction, internalized to peripheral neuron synapses, and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons to the CNS5. There, it inhibits neurotransmitter release by the specific degradation of the vesicle-secretory protein-Synaptobrevin6.

    Peptide Content: 100%
    Last update: 29/08/2021

    Tetanus Toxin (#T-150) is a highly pure, natural, and biologically active protein toxin.

    For research purposes only, not for human use
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