TRPC4 Blocking Peptide (#BLP-CC018) is the original antigen used for immunization during Anti-TRPC4 Antibody (#ACC-018) generation. The blocking peptide binds and ‘blocks’ Anti-TRPC4 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes:
- Western blot analysis of human PC3 Caucasian prostate adenocarcinoma (lanes 1 and 3) and human LNCaP prostate carcinoma (lanes 2 and 4) cell lysates:
- Expression of TRPC4 in mouse cerebellumImmunohistochemical staining of mouse cerebellum frozen sections with Anti-TRPC4 Antibody (#ACC-018). A. TRPC4 (red) appears in Purkinje cells (arrows) and in the molecular (Mol) layer. B. Staining with mouse anti-parvalbumin (PV) in the same brain section. C. Confocal merge of TRPC4 and PV demonstrates partial co-localization in the Purkinje and the molecular layers.
- (C)KEKHAHEEDSSIDYDL, corresponding to amino acid residues 943-958 of mouse TRPC4 (Accession Q9QUQ5).