Overview
Cat #: BLP-CC044
Type: Synthetic peptide
Form: Lyophilized powder
TRPM4 Blocking Peptide (#BLP-CC044) is the original antigen used for immunization during Anti-TRPM4 Antibody (#ACC-044) generation. The blocking peptide binds and ‘blocks’ Anti-TRPM4 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of rat lung (lanes 1 and 5), rat heart membrane (lanes 2 and 6), mouse brain membrane (lanes 3 and 7) and ms1 mouse pancreas cells lysate (lanes 4 and 8):1-4. Anti-TRPM4 Antibody (#ACC-044), (1:200).
5-8. Anti-TRPM4 Antibody, preincubated with TRPM4 Blocking Peptide (#BLP-CC044). - Expression of TRPM4 in rat lungImmunohistochemical staining of rat lung paraffin embedded sections using Anti-TRPM4 Antibody (#ACC-044), (1:100). TRPM4 is expressed in the respiratory epithelium of the bronchioli (arrows). Hematoxilin is used as the counterstain.
Properties
Sequence
Accession (Uniprot) Number Q8TD43
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.