TRPM4 Blocking Peptide (#BLP-CC044) is the original antigen used for immunization during Anti-TRPM4 Antibody (#ACC-044) generation. The blocking peptide binds and ‘blocks’ Anti-TRPM4 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat lung (lanes 1 and 5), rat heart membrane (lanes 2 and 6), mouse brain membrane (lanes 3 and 7) and ms1 mouse pancreas cells lysate (lanes 4 and 8):
- Expression of TRPM4 in rat lungImmunohistochemical staining of rat lung paraffin embedded sections using Anti-TRPM4 Antibody (#ACC-044), (1:100). TRPM4 is expressed in the respiratory epithelium of the bronchioli (arrows). Hematoxilin is used as the counterstain.
- EKEQSWIPKIFKK(C), corresponding to amino acid residues 5-17 of human TRPM4 (Accession Q8TD43).