TRPM5 Blocking Peptide (#BLP-CC045) is the original antigen used for immunization during Anti-TRPM5 Antibody (#ACC-045) generation. The blocking peptide binds and ‘blocks’ Anti-TRPM5 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat lung membrane (lanes 1 and 2), rat testes (lanes 3 and 4), mouse ms1 pancreas cells (lanes 5 and 7) and human LNCaP prostate cell (lanes 6 and 8) lysates:
- Expression of TRPM5 in rat tongueImmunohistochemical staining of rat tongue paraffin embedded sections using Anti-TRPM5 Antibody (#ACC-045), (1:100). TRPM5 is expressed in discrete groups of cells deep in the sulcus of the papillae. Hematoxilin is used as the counterstain.
- Expression of TRPM5 in rat testesImmunohistochemical staining of rat epididymis paraffin embedded sections using Anti-TRPM5 Antibody (#ACC-045), (1:100). TRPM5 is expressed in principal cells within the epididymal epithelium (arrows). Hematoxilin is used as the counterstain.
- GSGKKRGKFVKVPS(C), corresponding to amino acid residues 32-45 of mouse TRPM5 (Accession Q9JJH7).