Overview
Type: Synthetic peptide
Form: Lyophilized powder
TRPV5 Blocking Peptide (#BLP-CC035) is the original antigen used for immunization during Anti-TRPV5 Antibody (#ACC-035) generation. The blocking peptide binds and ‘blocks’ Anti-TRPV5 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat kidney (lanes 1 and 3) and human PC3 prostate cancer cell line (lanes 2 and 4) lysates:1,2. Anti-TRPV5 Antibody (#ACC-035), (1:200).
3,4. Anti-TRPV5 Antibody, preincubated with TRPV5 Blocking Peptide (#BLP-CC035).
Expression of TRPV5 in rat kidneyImmunohistochemical staining of rat kidney paraffin embedded section using Anti-TRPV5 Antibody (#ACC-035), (1:100). TRPV5 is expressed in collecting ducts (CD) in the renal cortex. Note that both proximal tubules (PT) and distal tubules (DT) are negative. Hematoxilin is used as the counterstain.- Mouse kidney sections (Loh, N.Y. et al. (2013) PLoS ONE 8, e55412.).
Properties
Sequence
- (C)GLNLSEGDGEEVYHF, corresponding to amino acid residues 715-729 of human TRPV5 (Accession Q8NDW5).
Accession (Uniprot) Number Q8NDW5
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
