Anti-TRPV5 Antibody

Transient receptor potential cation channel subfamily V member 5, Calcium transport protein 2, CaT2, Epithelial calcium channel 1, ECaC1, OTRPC3
    Cat #: ACC-035
    Alternative Name Transient receptor potential cation channel subfamily V member 5, Calcium transport protein 2, CaT2, Epithelial calcium channel 1, ECaC1, OTRPC3
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)GLNLSEGDGEEVYHF, corresponding to amino acid residues 715-729 of human TRPV5 (Accession Q9NQA5). Intracellular, C-terminus.
    • Anti-TRPV5 Antibody
    Accession (Uniprot) Number Q8NDW5
    Gene ID 56302
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat - 11/15 amino acid residues identical; rabbit - 13/15 amino acid residues identical.
    RRID AB_2039793.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, wb
    May also work in: ifc*, ip*
    Western blot
    • Anti-TRPV5 Antibody
      Western blot analysis of rat kidney (lanes 1 and 3) and PC3 cell line (lanes 2 and 4) lysates:
      1,2. Anti-TRPV5 Antibody (#ACC-035), (1:200).
      3,4. Anti-TRPV5 Antibody, preincubated with the negative control antigen.
    Immunohistochemistry
    • Anti-TRPV5 Antibody
      Expression of TRPV5 in rat kidney
      Immunohistochemical staining of rat kidney paraffin embedded section using Anti-TRPV5 Antibody (#ACC-035), (1:100). TRPV5 is expressed in collecting ducts (CD) in the renal cortex. Note that both proximal tubules (PT) and distal tubules (DT) are negative. Hematoxilin is used as the counterstain.
    • Mouse kidney sections (Loh, N.Y. et al. (2013) PLoS ONE 8, e55412.).
    Immunocytochemistry
    • Anti-TRPV5 Antibody
      Expression of TRPV5 in PC3 cells
      Immunocytochemical staining of paraformaldehyde-fixed PC-3 cells with Anti-TRPV5 Antibody (#ACC-035), (1:100), followed by goat anti-rabbit-AlexaFluor-555 secondary antibody (A). B. Same as in (A) but pre-treated with TRPV5 peptide antigen (1:20). Hoechst 33342 is used as the counterstain (blue).
    References
    1. Montell, C. et al. (2002) Mol. Cell. 9, 229.
    2. Clapham, D.E. (2003) Nature 426, 517.
    3. Moran, M.M. et al. (2004) Curr. Opin. Neurobiol. 14, 362.
    4. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591.
    5. Gunthorpe, M.J. et al. (2002) Trends. Pharmacol. Sci. 23, 183.
    6. Hoenderop, J.G. et al. (2003) Pflugers. Arch. 446, 304.
    7. Peng, J.B. et al. (2003) J. Physiol. 551, 729.
    Scientific background

    TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 According to IUPHAR, the TRP family is comprised of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP).1-4 The TRPV subfamily consists of six members, TRPV1-6.5

    TRPV5 (ECaC) and TRPV6 (CaT1) are unique members of the TRP family. Both are epithelial Ca2+ channels, form constitutively open channels, share high degree of homology (about 66%) with differences in the N- and C-terminus. TRPV6 and TRPV5 are expressed in a 1,25-dihydroxyvitamin D3 dependent manner. In contrast to other members of the TRP family, TRPV5 and TRPV6 are highly Ca2+ selective and are expressed in Ca2+ transporting epithelia where they are assumed to have an important role in Ca2+ reabsorption.6,7 TRPV5 is preferentially expressed in the kidney.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 10/02/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human TRPV5. Anti-TRPV5 Antibody (#ACC-035) can be used in western blot, immunocytochemistry and immunohistochemistry applications. It has been designed to recognize TRPV5 from human, mouse and rat samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Western blot citations
    1. Mouse kidney lysate.
      Wang, L. et al. (2015) J. Am. Soc. Nephrol. 26, 2678.
    Immunohistochemistry citations
    1. Mouse kidney sections.
      Alexander, R.T. et al. (2015) Am. J. Physiol. 309, F604.
    2. Mouse kidney sections.
      Loh, N.Y. et al. (2013) PLoS ONE 8, e55412.
    More product citations
    1. Li, S. et al. (2010) Mol. Biol. Rep. 37, 1287.
    2. Irnaten, M. et al. (2009) Steroids 74, 642.
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