TRPV6 Blocking Peptide (#BLP-CC036) is the original antigen used for immunization during Anti-TRPV6 Antibody (#ACC-036) generation. The blocking peptide binds and ‘blocks’ Anti-TRPV6 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of Human PC-3 prostate cancer cell lysate:
- Expression of TRPV6 in rat DRGImmunohistochemical staining of rat dorsal root ganglion (DRG) using Anti-TRPV6 Antibody (#ACC-036), (1:100). A. TRPV6 channel (red) in DRG neurons. B. Staining with mouse anti-Parvalbumin (green) in the same DRG section. C. Confocal merge of TRPV6 and Parvalbumin demonstrates colocalization (arrows).
- (C)NRGLEDGESWEYQI, corresponding to amino acid residues 712-725 of human TRPV6 (Accession Q9H1D0).