Overview
Type: Synthetic peptide
Form: Lyophilized powder
TRPV6 Blocking Peptide (#BLP-CC036) is the original antigen used for immunization during Anti-TRPV6 Antibody (#ACC-036) generation. The blocking peptide binds and ‘blocks’ Anti-TRPV6 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of Human PC-3 prostate cancer cell lysate:1. Anti-TRPV6 Antibody (#ACC-036), (1:200).
2. Anti-TRPV6 Antibody, preincubated with TRPV6 Blocking Peptide (#BLP-CC036).
Expression of TRPV6 in rat DRGImmunohistochemical staining of rat dorsal root ganglion (DRG) using Anti-TRPV6 Antibody (#ACC-036), (1:100). A. TRPV6 channel (red) in DRG neurons. B. Staining with mouse anti-Parvalbumin (green) in the same DRG section. C. Confocal merge of TRPV6 and Parvalbumin demonstrates colocalization (arrows).
Properties
Sequence
- (C)NRGLEDGESWEYQI, corresponding to amino acid residues 712-725 of human TRPV6 (Accession Q9H1D0).
Accession (Uniprot) Number Q9H1D0
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
