Anti-TRPV6 Antibody

Epithelial calcium channel 2, ECaC2, Calcium transport protein 1, CaT1, CaT-like, CaT-L, Transient receptor potential cation channel subfamily V member 6
    Cat #: ACC-036
    Alternative Name Epithelial calcium channel 2, ECaC2, Calcium transport protein 1, CaT1, CaT-like, CaT-L, Transient receptor potential cation channel subfamily V member 6
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)NRGLEDGESWEYQI, corresponding to amino acid residues 712-725 of human TRPV6 (Accession Q9H1D0). Intracellular, C-terminus.
    • Anti-TRPV6 Antibody
    Accession (Uniprot) Number Q9H1D0
    Gene ID 55503
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse - 13/14 amino acid residues identical.
    RRID AB_2039791.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.75 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, ip, wb
    May also work in: ifc*
    Western blot
    • Anti-TRPV6 Antibody
      Western blot analysis of PC-3 cell lysate:
      1. Anti-TRPV6 Antibody (#ACC-036), (1:200).
      2. Anti-TRPV6 Antibody, preincubated with the negative control antigen.
    Immunoprecipitation
    Immunohistochemistry
    • Anti-TRPV6 Antibody
      Expression of TRPV6 in rat DRG
      Immunohistochemical staining of rat dorsal root ganglion (DRG) using Anti-TRPV6 Antibody (#ACC-036), (1:100). A. TRPV6 channel (red) in DRG neurons. B. Staining with mouse anti-Parvalbumin (green) in the same DRG section. C. Confocal merge of TRPV6 and Parvalbumin demonstrates colocalization (arrows).
    Immunocytochemistry
    • Anti-TRPV6 Antibody
      Expression of TRPV6 in PC-3 cells
      Immunocytochemical staining of paraformaldehyde-fixed PC-3 cells with Anti-TRPV6 Antibody (#ACC-036), (1:100) followed by goat anti-rabbit-AlexaFluor-555 secondary antibody (A) or with a mixture of Anti-TRPV6 Antibody and TRPV6 peptide antigen (1:20) (B). Hoechst 33342 is used as the counterstain (blue).
    References
    1. Montell, C. et al. (2002) Mol. Cell. 9, 229.
    2. Clapham, D.E. (2003) Nature 426, 517.
    3. Moran, M.M. et al. (2004) Curr. Opin. Neurobiol. 14, 362.
    4. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591.
    5. Gunthorpe, M.J. et al. (2002) Trends. Pharmacol. Sci. 23, 183.
    6. Hoenderop, J.G. et al. (2003) Pflugers. Arch. 446, 304.
    7. Peng, J.B. et al. (2003) J. Physiol. 551, 729.
    8. Fixemer, T. et al. (2003) Oncogene 22, 7858.
    9. Wissenbach, U. et al. (2001) J. Biol. Chem. 276, 19461.
    Scientific background

    TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP).1-4 The TRPV subfamily consists of six members, TRPV1-6.5

    TRPV6 (CaT1) and TRPV5 (ECaC) are unique members of the TRP family. Both are epithelial calcium channels, form constitutively open channels, share high degree of homology (about 66%) with differences in the N and C-terminus. TRPV6 and TRPV5 are expressed in a 1,25-dihydroxyvitamin D3 dependent manner. In contrast to other members of the TRP family, TRPV6 and TRPV5 are highly Ca2+ selective and are expressed in Ca2+ transporting epithelia where they are assumed to have an important role in Ca2+ reabsorption. TRPV6 is highly expressed in the placenta, small intestine and kidney.6,7

    While not being expressed in benign prostate tissue, TRPV6 was found to be upregulated in prostate cancer tissue and correlation between expression and tumor grade was shown. Finally, TRPV6 was suggested as prognostic marker and as a promising candidate for therapeutic strategies.8,9

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title: Anti-TRPV6 Antibody Expression of TRPV6 in prostate cancer.Immunohistochemical staining of human prostate tissue using Anti-TRPV6 Antibody (#ACC-036). Very low expression of TRPV6 is observed in normal (right panel) and benign hyperplasia (middle panel) as opposed to significant expression in prostate adenocarcinoma.Adapted from Raphael, M. et al. (2014) Proc. Natl. Acad. Sci. U.S.A. 111, E3870.
    Last update: 10/02/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the human TRPV6. Anti-TRPV6 Antibody (#ACC-036) can be used in western blot, immunoprecipitation, immunocytochemistry and immunohistochemistry applications. It has been designed to recognize TRPV6 from human, rat, and mouse samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Immunoprecipitation citations
    1. Human LNCaP cells.
      Lallet-Daner, H. et al. (2009) Oncogene 28, 1792.
    Immunohistochemistry citations
    1. Rat epididymis sections.
      Gao, da Y. et al. (2016) J. Gen. Physiol. 148, 161.
    Immunocytochemistry citations
    1. Human LNCap cells.
      Lehen’kyi, V. et al. (2011) PLoS ONE 6, e16856.
    More product citations
    1. Raphael, M. et al. (2014) Proc. Natl. Acad. Sci. U.S.A. 111, E3870.
    2. Kim, S.Y. et al. (2013) Cell Calcium 53, 102.
    3. Hatano, R. et al. (2012) Kidney Int. 83, 41.
    4. Sprekeler, N. et al. (2012) Reprod. Biol. Endocrinol. 10, 66.
    5. Yang, S.S. et al. (2010) Endocrinology 151, 1829.
    6. Choi, Y. et al. (2009) Biol. Reprod. 81, 1122.
    7. Lee, B.M. et al. (2009) Reprod. Biol. Endocrinol. 7, 1.
    8. Semenova, S.B. et al. (2009) Am. J. Physiol. 296, C1098.
    9. Irnaten, M. et al. (2008) Cell Calcium 44, 441.
    10. Lehen’kyi, V. et al. (2007) Oncogene 26, 7380.
    11. Lehen’kyi, V. et al. (2007) J. Biol. Chem. 282, 22582.
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