Overview
Type: Synthetic peptide
Form: Lyophilized powder
VGLUT1 Blocking Peptide (#BLP-GC035) is the original antigen used for immunization during Anti-VGLUT1 Antibody (#AGC-035) generation. The blocking peptide binds and ‘blocks’ Anti-VGLUT1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat cortex lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1,2. Anti-VGLUT1 Antibody (#AGC-035), (1:200).
3,4. Anti-VGLUT1 Antibody, preincubated with VGLUT1 Blocking Peptide (#BLP-GC035).
Expression of VGLUT1 in rat cortexImmunohistochemical staining of perfusion-fixed rat brain frozen sections using Anti-VGLUT1 Antibody (#AGC-035), (1:400), followed by goat-anti-rabbit-Alexa-488. VGLUT1 staining (green) appears in neurons of the pyramidal layer of the parietal cortex (arrows). DAPI is used as the nuclear counterstain (blue).
Western blot analysis of rat brain cortex lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1-2. Guinea Pig Anti-VGLUT1 Antibody (#AGC-035-GP), (1:400).
3-4. Guinea Pig Anti-VGLUT1 Antibody, preincubated with VGLUT1 Blocking Peptide (#BLP-GC035).
Expression of VGLUT1 in mouse dorsal root nucleus (DRN).Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Guinea Pig Anti-VGLUT1 Antibody (#AGC-035-GP), (1:300), followed by goat anti-guinea pig-Alexa Fluor-594. A. VGLUT1 immunoreactivity (red) appears in in DRN cells (arrows). B. Pre-incubation of the antibody with VGLUT1 Blocking Peptide (#BLP-GC035), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of VGLUT1 in rat spinal cord.Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections with Guinea Pig Anti-VGLUT1 Receptor 1 Antibody (#AGC-035-GP), (1:300), followed by goat anti-guinea pig-Alexa Fluor-594. A. VGLUT1 immunoreactivity (red) appears in layers 1-2 of spinal dorsal horn (vertical arrows) and in the dorsal root (horizontal arrow). B. Pre-incubation of the antibody with VGLUT1 Blocking Peptide (#BLP-GC035), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)EFRQEEFRKLAGR, corresponding to amino acid residues 2-14 of rat VGLUT1 (Accession Q62634).
Accession (Uniprot) Number Q62634
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
