Name: α-Bungarotoxin-Biotin
Brand: Alomone Labs
SKU: B-100-B

Cat #: B-100-B

Alternative Name Long neurotoxin 1, α-Bgtx, α-BuTX

Lyophilized Powder yes

Bioassay Tested

Origin Modified natural protein isolated from Bungarus multicinctus (Many-banded krait).

MW: ~9030 Da

Purity: >95% (HPLC)

Form Lyophilized powder

Effective concentration 100-200 nM

Sequence Biotin-IVCHTTATSPISAVTCPPGENLCYRKMWCDAFCSSRGKVVELGCAATCPSKKPYEEVTCCSTDKCNPHPKQRPG

Modifications Disulfide bonds between Cys³-Cys²³, Cys¹⁶-Cys⁴⁴, Cys²⁹-Cys³³, Cys⁴⁸-Cys and Cys⁶⁰-Cys⁶⁵

Label LC-Biotin. The extent of labeling is 1-3 molecules of biotin per molecule α-Bungarotoxin.

Structure

Activity α-Bungarotoxin blocks postsynaptic neuromuscular transmission via competitive inhibition of nicotinic ACh receptors (nAChRs), thereby preventing the depolarizing action on postsynaptic membranes and blocking neuromuscular transmission. Selective for α7 receptors (IC₅₀ value of 1.6 nM) and α3/β4 receptors (IC₅₀ value of >3 μM)^1,2. The toxin also blocks GABA(A) receptor subtypes³.

References-Activity

Wilson, S.P. and Kirshner, N. (1977) J. Neurochem. 28, 687.
Garcia-Guzman, M. et al. (1995) Eur. J. Neurosci. 7, 647.
McCann, C.M. et al. (2006) Proc. Natl. Acad. Sci. U.S.A. 103, 5149.

Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions Up to two weeks at 4°C or three months at -20°C.

Our bioassay

Bungarotoxin binding sites co-localize with GABAergic neurons expressing parvalbumin in mouse hippocampal CA1 region.
A. Free floating mouse brain sections were incubated with α-Bungarotoxin-Biotin (#B-100-B), (1:10,000) followed by streptavidin-Alexa 488 (green). B. Same sections were stained with anti-parvalbumin, followed by goat anti-mouse labeled with Texas red isothiocyanate (TRITC). C. Merge of A and B demonstrates sites of colocalization (vertical arrows) confirming reports that a subset of hippocampal interneurons express nAChR α7. DAPI is used as the counterstain.
Alomone Labs α-Bungarotoxin-Biotin inhibits muscle nAChR channels heterologously expressed in Xenopus oocytes.
A. Time course of muscle nAChR (α1/β1/γ/δ) current recording. Membrane potential was held at -80 mV and stimulated by perfused with a solution containing 100 µM ACh and 3 μM PNU-120596 every 100 sec. 200 nM α-Bungarotoxin-Biotin (#B-100-B) was applied (green) during the ACh application for 5.5 min and inhibited channel current, as indicated. B. Superimposed examples of muscle nAChR current in the absence (black) and presence (green) of 200 nM α-Bungarotoxin-Biotin (taken from the experiment in A).

Scientific background

α-Bungarotoxin isoform A31 is a 74 amino acid peptidyl toxin isolated from the venom of the banded krait snake, Bungarus multicinctus¹.

α-Bungarotoxin blocks postsynaptic neuromuscular transmission via competitive inhibition of nicotinic ACh receptors (nAChRs) with an IC₅₀ of 3.5 x 10^-10 M, thereby preventing the depolarizing action on postsynaptic membranes and blocking neuromuscular transmission².

The toxin is selective for α7 receptors (IC₅₀ value of 1.6 nM) and α3/β4 receptors (IC₅₀ value of >3 µM)^3,4.

α-Bungarotoxin also binds to and blocks a subset of GABA_A receptors (GABA_ARs) that contain the GABA_AR β3 subunit. In particular, α-Bungarotoxin blocks GABA_ARs that contain interfaces between adjacent β3 subunits⁵.

Target α7, α1/β1/γ/δ nAChR, GABA(A) receptor subtypes

Peptide Content: 100%

Lyophilized Powder

Last update: 11/12/2022

For research purposes only, not for human use

Specifications

Citations

Applications

Scientific Background

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Order Summary

Certificate of Analysis

α-Bungarotoxin-Biotin

Specifications

Citations

Applications

Scientific Background

Resources

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