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A Potent Activator of KCa3.1 and KCa2 Channels

Cat #: E-150
Alternative Name 1-Ethyl-2-benzimidazolinone
Lyophilized Powder yes
  • Bioassay Tested
  • Source Synthetic
    MW: 162.19
    Purity: >97%
    Effective concentration 5-100 μM.
    Chemical name 3-ethyl-1H-benzimidazol-2-one.
    Molecular formula C9H10N2O.
    CAS No.: 10045-45-1
    PubChem CID 82320
    Activity 1-EBIO is an activator of KCa3.1 and KCa2 channel currents1.
    1. Waschk, D.E. et al. (2011) Am. J. Physiol. Cell. Physiol. 300, C843.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility 250 mM DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to four weeks at 4°C or three months at -20°C.
    Our bioassay
    • Alomone Labs 1-EBIO activates KCa3.1 currents expressed in HEK 293T cells.
      Alomone Labs 1-EBIO activates KCa3.1 currents expressed in HEK 293T cells.
      A. Time course of KCa3.1 current amplitude and its activation by 50 µM 1-EBIO (#E-150). Currents were elicited by application of voltage ramp from -120 mV to +60 mV (150 msec), delivered every 10 seconds, holding potential of -80mV. B. Superimposed example traces of current responses before and during perfusion of 50 µM 1-EBIO as indicated.
    References - Scientific background
    1. Wong, R. et al. (2014) J. Neurosci. 34, 13371.
    2. Coleman, N. et al. (2014) Mol. Pharmacol. 86, 342.
    3. Strøbæk, D. et al. (2013) Br. J. Pharmacol. 168, 432.
    Scientific background

    1-EBIO is a potent KCa2/3 channel activator that activates the channels by increasing the Ca2+ sensitivity by stabilizing the interaction between calmodulin and the channels1,2, thus shifting the activation curve of the channels to the left. 1-EBIO activates KCa3.1 with an EC50 value of around 30 μM and all three KCa2 channels with EC50 value around 300 μM2.

    The Ca2+-activated K+ channel, KCa3.1, plays a major role in the controlled expression of essential genes that are responsible for several physiological processes including- membrane potential, cell volume proliferation, migration and production of inflammatory mediators by participating in the regulation of calcium entry. A highly regulated and sustained increase in cytosolic Ca2+ is critical in these processes making KCa3.1 a potential target for therapy of several disease1,3.

    Target KCa3.1, KCa2 channels
    Last update: 06/11/2022

    1-EBIO (#E-150) is a highly pure, synthetic, and biologically active compound.

    For research purposes only, not for human use
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