Anti-KCNN1 (KCa2.1, SK1) Antibody

SKCa1, Small conductance calcium-activated potassium channel protein 1
    Cat #: APC-039
    Alternative Name SKCa1, Small conductance calcium-activated potassium channel protein 1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    May also work in: h*
      • Peptide (C)DRPGSGKPPTVSHRLGHRR corresponding to amino acid residues 75-93 of rat KCNN1 (Accession P70606). Intracellular, N-terminal part.
        Anti-KCNN1 (KCa2.1, SK1) Antibody
    Accession (Uniprot) Number P70606
    Gene ID 54261
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 17/19 amino acid residues identical; human - 12/19 amino acid residues identical.
    RRID AB_2039957.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.6 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, wb
    May also work in: ifc*, ip*
      • Anti-KCNN1 (KCa2.1, SK1) Antibody
        Western blot analysis of rat brain cortex: 
        1. Anti-KCNN1 (KCa2.1, SK1) Antibody (#APC-039), (1:200).  
        2. Anti-KCNN1 (KCa2.1, SK1) Antibody, preincubated with the negative control antigen.
        Mouse isolated mouse atria and HL-1 cell lysate (1:200) (Yi, F. et al. (2015) J. Biol. Chem. 290, 7016.).
      • Mouse kidney sections (1:200) (Li, Y. et al. (2016) PLoS ONE 11, e0155006.).
      • Mouse CCDcl1 cells (1:100) (Li, Y. et al. (2016) PLoS ONE 11, e0155006.).
    References
      • KCa2.1 is a member of the Ca2+-activated K+ channel family with small conductance that includes KCa2.2 (SK2) and KCa2.3 (SK3). The channel is voltage insensitive and is activated by intracellular Ca2+ in the submicromolar range. It has a similar topology to that of voltage-dependent K+ channels (KV channels), that is, six transmembrane domains and intracellular N- and C-termini. 

        KCa2.1 channel expression is roughly confined to neuronal tissue, although there are some indications that it may be present in non-neuronal tissues as well.  

        The sensitivity of KCa2.1 to the bee venom toxin Apamin (#STA-200) is a matter of some controversy. Expression of the human KCa2.1 in mammalian cell lines showed that most cells produce channels that can be blocked by Apamin. However, expression of KCa2.1 in Xenopus oocytes produced channels that were mostly insensitive to Apamin. Interestingly, the rat KCa2.1 is unable to form functional channels when expressed alone in either cell lines or oocytes.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-KCNN1 (KCa2.1, SK1) Antibody (#APC-039) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize KCNN1 from human, rat, and mouse samples.

    For research purposes only, not for human use
    Citations
      • Mouse brain lysate.
        Deng, P.Y. et al. (2019) J. Neurosci. 39, 28.
      • Rat brain lysate (1:500).
        Larson, R.A. et al. (2015) Am. J. Physiol. 308, H1547.
      • Mouse isolated mouse atria and HL-1 cell lysate (1:200).
        Yi, F. et al. (2015) J. Biol. Chem. 290, 7016.
      • Canine LA and PV cardiomyocytes (1:200).
        Qi, X.Y. et al. (2014) Circulation 129, 430.
      • Mouse kidney sections (1:200).
        Li, Y. et al. (2016) PLoS ONE 11, e0155006.
      • Mouse CCDcl1 cells (1:100).
        Li, Y. et al. (2016) PLoS ONE 11, e0155006.
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