Every lot is tried & tested in a relevant biological assay.
- Alomone Labs α-Conotoxin RgIA inhibits α7 nAChR heterologously expressed in Xenopus oocytes.A. Time course of α-Conotoxin RgIA (#STC-010) action on α7 nAChR currents, elicited every 50 sec. by a transient application of 200 µM ACh + 0.3 µM PNU-120596, while membrane potential was held at -80 mV. Application of 1 µM (green) and 5 μM (magenta) α-Conotoxin RgIA significantly inhibits the currents.
B. Superimposed traces of α7 nAChR currents upon application of control, 1 µM (green) and 5 μM (magenta) α-Conotoxin RgIA (taken from the recording in A).
- Ellison, M. et al. (2006) Biochemistry, 45, 1511.
- Ellison, M. et al. (2008) J Mol. Biol., 377, 1216.
- Callaghan, B. et al. (2008) J Neurosci., 28, 10943.
- Azam, L. et al. (2009) Acta Pharmacol. Sin., 30, 771.
- Hone, A. J. et al. (2018) FEBS Lett., 592, 1045.
- Vincler, M. et al. (2006) PNAS, 103, 17780.
- Vincler, M. et al. (2007) Expert Opin. Ther. Targets, 11, 891.
- Pacini, A. et al. (2016) Exp. Neurol., 282, 37.
α-conotoxin RgIA (RgIA) is a 13 amino acid peptidyl toxin cloned from a genomic DNA library of the marine worm-hunting sea snail, Conus regius1. RgIA belongs to the α4/3 subfamily of conotoxins (i.e., a family of peptides with four amino acids in the first loop and three in the second loop) and is a potent and selective antagonist of the α9α10 nicotinic acetylcholine receptor (nAChR) subtype, which also shows a weak activity towards α7 nAChR1,2. RgIA was also shown to inhibit high-voltage-activated (HVA) calcium channel currents in rat dorsal root ganglion (DRG) neurons3.
The nAChRs are acetylcholine-gated ion channels. Given the important physiological roles of nAChRs in pain, inflammation, nicotine addiction, Alzheimer's disease, and Parkinson's disease, specific targeting of the relevant nAChR subtypes is an attractive pharmaceutical strategy. α-conotoxins are among the most promising drug development leads for treating these diseases4,5. RgIA was shown to be an effective analgesic agent in a rat model of nerve injury and also reduced the immune response contributing to peripheral nerve damage6,7. Furthermore, RgIA was shown to prevent neuropathic pain induced by oxaliplatin treatment8.
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Alpha-conotoxin RgIA (#STC-010) is a highly pure, synthetic, and biologically active peptide toxin.
- Anti-CACNA1B (CaV2.2) Antibody (#ACC-002)
- Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular) Antibody (#ANC-007)
- Anti-Nicotinic Acetylcholine Receptor α10 (CHRNA10) Antibody (#ANC-010)
- Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular)-ATTO Fluor-633 Antibody (#ANC-007-FR)
- Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular)-FITC Antibody (#ANC-007-F)