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Anti-Nicotinic Acetylcholine Receptor α10 (CHRNA10) Antibody

nAChR α10, Cholinergic receptor nicotinic alpha 10, Neuronal acetylcholine receptor subunit alpha-10

Cat #: ANC-010
Alternative Name nAChR α10, Cholinergic receptor nicotinic alpha 10, Neuronal acetylcholine receptor subunit alpha-10
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide (C)RRHEDWKRLARVMDR, corresponding to amino acid residues 411-425 of rat nAChRα10 (Accession Q9JLB5). 2nd intracellular loop.
Accession (Uniprot) Number Q9JLB5
Gene ID 64574
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – identical; human – 13/15 amino acid residues identical.
RRID AB_2756677.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
References
  1. Lustig, L.R. (2006) Anat. Rec. A Discov. Mol. Cell. Evol. Biol. 288, 4.
  2. Gomez-Casati, M.E. et al. (2009) J. Assoc. Res. Otolaryngol. 10, 221.
Scientific background

Ligand-gated ion channels are expressed throughout the brain where they mediate fast synaptic transmission. The nicotinic acetylcholine receptors (nAChRs) represent an important subcategory of these ligand-gated ion channels; nAChRs are multisubunit, neurotransmitter-gated receptors of the cholinergic system.

All nicotinic acetylcholine receptors are pentamers composed of homologous protein subunits, each subunit spanning the plasma membrane four times. There are 10 α-subunits (α1–α10), 4 β-subunits (β1–β4), and γ-, δ-, and ϵ-subunits, all with shared homology. Various combinations of subunits form subtypes of the acetylcholine receptor. α-subunits of CHRNA10 consist of a N-terminal extracellular domain that contains the ACh binding region. There are four hydrophilic membrane-spanning domains (often labeled as M1–4) and an intracellular loop between M3 and M4.

The α10 gene encoding the CHRNA10 subunit is mostly expressed in outer hair cells in the ear. To this day, the α10 receptor has only been shown to form a functional receptor when combined with α9. As compared to expressed homomeric α9 receptors, the heteromeric α9α10 receptors display faster and more extensive agonist-mediated desensitization, a distinct current-voltage relationship, and a biphasic response to changes in extracellular Ca2+ ions. The primary role identified for CHRNA10 function within the inner ear is related to efferent auditory neuronal activity1.

CHRNA10 knockout does not cause major alterations either in the functional expression of other voltage-gated and/or Ca2+-sensitive K+ channels or in the electrical properties of the cochlear inner hearing cells2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 12/08/2021

Anti-Nicotinic Acetylcholine Receptor α10 (CHRNA10) Antibody (#ANC-010) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize nAChRα10 from rat, mouse, and human samples.

For research purposes only, not for human use
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