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Anti-Alpha 1 Na+/K+ ATPase Antibody

ATP1A1, Sodium/potassium ATPase catalytic subunit alpha-1, Sodium pump subunit alpha-1

Cat #: ANP-001
Alternative Name ATP1A1, Sodium/potassium ATPase catalytic subunit alpha-1, Sodium pump subunit alpha-1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)RDKYEPAAVSEHGD, corresponding to amino acid residues 7- 20 of rat ATP1A1 (Accession P06685). Intracellular, N-terminus.
Accession (Uniprot) Number P06685
Gene ID 24211
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, dog, pig – identical; Human- 13/14 amino acid residues identical.
RRID AB_2341003.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:
    1,2. Anti-Alpha 1 Na+/K+ ATPase Antibody (#ANP-001), (1:200).
    3,4. Anti-Alpha 1 Na+/K+ ATPase Antibody, preincubated with Alpha 1 Na+/K+ ATPase Blocking Peptide (#BLP-NP001).
  • Western blot analysis of human Colo-205 colon adenocarcinoma cell lysate:
    Western blot analysis of human Colo-205 colon adenocarcinoma cell lysate:
    1. Anti-Alpha 1 Na+/K+ ATPase Antibody (#ANP-001), (1:200).
    2. Anti-Alpha 1 Na+/K+ ATPase Antibody, preincubated with Alpha 1 Na+/K+ ATPase Blocking Peptide (#BLP-NP001).
    Note: Samples should be heated up to 37°C. Do not boil samples.
Immunohistochemistry
  • Expression of Na+/K+ ATPase α1 (ATP1A1) in rat kidney
    Expression of Na+/K+ ATPase α1 (ATP1A1) in rat kidney
    Immunohistochemical staining of paraffin embedded rat kidney sections using Anti-Alpha 1 Na+/K+ ATPase Antibody (#ANP-001), (1:200). ATP1A1 staining (brown) is present in collecting ducts and thick portions of the Henle loop in the renal medulla. Hematoxilin is used as the counterstain.
  • Expression of Na+/K+ ATPase α1 (ATP1A1) in mouse brain
    Expression of Na+/K+ ATPase α1 (ATP1A1) in mouse brain
    Immunohistochemical staining of immersion-fixed, free floating mouse brain frozen sections using Anti-Alpha 1 Na+/K+ ATPase Antibody (#ANP-001), (1:400). ATP1A1 staining (red) is apparent in the hippocampal dentate gyrus in neuronal soma (vertical arrows) and in astrocytes (horizontal arrow). DAPI (blue) is used to visualize cell nuclei.
References
  1. Goldin, S.M. et al. (1977) J. Biol. Chem. 252, 5630.
  2. Jorgensen, P. L. and Andersen, J.P. (1988) J. Membr. Biol. 103, 95.
  3. Pietrini, G. et al. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 8414.
  4. Guidotti, G. et al. (1990) Chemtracts: Biochem. Mol. Biol. 1, 447.
  5. Yoon, K. L. and Guidotti, G. (1994) J. Biol. Chem. 269, 28249.
  6. Ji, L. et al. (2009) Life Sci. 85, 788.
Scientific background

Na+/K+-ATPase, a membrane protein located on the plasma membrane of all animal cells, is involved in maintaining the Na+ and K+ gradients across the cell membrane1. It is ubiquitously expressed in neurons and helps maintain normal neuronal function2. The enzyme is composed of a catalytic α chain of 112 kDa. and α glycosylated β chain of 40–50 kDa.3. There are four isoforms of the α subunit (α1, α2, α3 and α4) and three isoforms of the β subunit (β1, β2 and β3) with different tissue distributions4.
 
The N-terminus is located on the cytoplasmic side of the membrane. There are four transmembrane segments in the first third of the polypeptide chain, a large cytoplasmic loop (LCL) comprising the second third of the chain and four to six transmembrane segments in the C-terminal third of the protein with the C-terminus also on the cytoplasmic side of the membrane5.
 
Na+/K+-ATPase abnormalities have been reported to be involved in several neurological diseases such as seizures, bipolar disorder, spongiform encephalopathy, and Alzheimer’s disease. Na+/K+-ATPase may also have implications in behavioral defects6.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 24/01/2021

Anti-Alpha 1 Na+/K+ ATPase Antibody (#ANP-001) is a highly specific antibody directed against an epitope of the rat ATP1A1. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize ATP1A1 from rat, mouse, and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Western blot citations
  1. HEK-293 cell lysate.
    Nissenkorn, A. et al. (2019) PLoS ONE 14, e0211901.
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