Anti-Alpha 3 Na+/K+ ATPase Antibody

ATPase Na+/K+ Transporting Subunit Alpha 3, Sodium-Potassium ATPase Catalytic Subunit Alpha-3, ATP1A3
  • New
Cat #: ANP-003
Sizes: 25 µl, 50 µl, 0.2 ml
  • Lyophilized Powder
  • Antigen Incl.
  • QC Tested
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)DKKDDKSSPKKSK, corresponding to amino acid residues 3 - 15 of rat Alpha 3 Na+/K+ ATPase (Accession P06687). Intracellular, N-terminus.
    Accession (Uniprot) Number P06687
    Gene ID 24213
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse – identical; human – 11/13 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
    May also work in: ic*, ifc*, ip*
    Western blot
    Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    1, 2. Anti-Alpha 3 Na+/K+ ATPase Antibody (#ANP-003), (1:200).
    3, 4. Anti-Alpha 3 Na+/K+ ATPase Antibody, preincubated with the control peptide antigen.
    Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:

    Anti-Alpha 3 Na+/K+ ATPase Antibody (#ANP-003), (1:200).
    2. Anti-Alpha 3 Na+/K+ ATPase Antibody, preincubated with the control peptide antigen.

    Immunohistochemistry
    Expression of Alpha 3 Na+/K+ ATPase in rat hippocampus
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Alpha 3 Na+/K+ ATPase Antibody (#ANP-003), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. Alpha 3 Na+/K+ ATPase staining (green) in the hippocampal dentate gyrus is detected in the granule layer (G), (vertical arrows) and in hilar interneurons (horizontal arrows). Cell nuclei are stained with DAPI (blue).
    References
    1. Johansson, M. et al. (2003) J. Clin. Endocrinol. Metab. 88, 2831.
    2. Schirinzi, T.  et al. (2018) Cerebellum. doi: 10.1007/s12311-018-0920-y.
    3. Holm, T.H. et al. (2016) Sci. Rep. 6, 31972.
    Scientific background

    Alpha 3 Na+/K+ ATPase (ATP1A3) subunit is responsible for maintaining the electrochemical gradient for Na+ and K+ across the plasma membrane. A low intracellular Na+ concentration in eukaryotic cells is maintained by the Na+/K+ ATPase protein. Na+/K+ ATPase exports three sodium ions for every two potassium ions imported1-3.

    In most cells Na+/K+ ATPase is distributed along the plasma membrane, whereas in epithelia the distribution is polarized in patterns specific for the type of epithelium. In the central nervous system (CNS), Alpha 3 Na+/K+ ATPase isoform is expressed in neurons1,3.

    The establishment of an electrochemical gradient for Na+ and K+ across the plasma membrane is vital for cell function, it determines cell resting membrane potentials and supports the electrical activity of excitable cells.  

    Autosomal dominant mutations of human gene are related to a wide spectrum of clinical conditions, including several defined syndromes as rapid-onset dystonia-parkinsonism, hyperactivity, epileptic seizures, optic atrophy, and sensorineural hearing loss2,3.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 15/04/2018

    Anti-Alpha 3 Na+/K+ ATPase Antibody (#ANP-003) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize ATP1A3 from rat, mouse, and human samples.

    For research purposes only, not for human use