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Anti-Neuroligin 3 (extracellular) Antibody

NLGN3, Gliotactin homolog, HNL3, NL3

Cat #: ANR-037
Alternative Name NLGN3, Gliotactin homolog, HNL3, NL3
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)SHITRRPNGKTWSTKR, corresponding to amino acid residues 656-671 of rat NLGN3 (Accession Q62889). Extracellular, N-terminus.
Accession (Uniprot) Number Q62889
Gene ID 171297
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human – identical.
RRID AB_2756689.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ifc*, ip*, lci*
Western blot
  • Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysate:
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysate:
    1,2. Anti-Neuroligin 3 (extracellular) Antibody (#ANR-037), (1:200).
    3,4. Anti-Neuroligin 3 (extracellular) Antibody, preincubated with Neuroligin 3 (extracellular) Blocking Peptide (#BLP-NR037).
Immunohistochemistry
  • Expression of Neuroligin-3 in rat cerebellum. 
    Expression of Neuroligin-3 in rat cerebellum. 
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Neuroligin 3 (extracellular) Antibody(#ANR-037), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Neuroligin-3 immunoreactivity (green) appears in in the granule layer (GL) and in some purkinje cells (arrows). B. Pre-incubation of the antibody with Neuroligin 3 (extracellular) Blocking Peptide (BLP-NR037), suppressed staining. Cell nuclei are stained with DAPI (blue). 
  • Expression of Neuroligin-3 in rat deep cerebellar nuclei. 
    Expression of Neuroligin-3 in rat deep cerebellar nuclei. 
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Neuroligin 3 (extracellular) Antibody(#ANR-037), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Neuroligin-3 immunoreactivity (green) appears in deep cerebellar nuclei forming neuronal outlines (arrows). B. Pre-incubation of the antibody with Neuroligin 3 (extracellular) Blocking Peptide (BLP-NR037), suppressed staining. Cell nuclei are stained with DAPI (blue). 
References
  1. Hu, X. et al. (2015) Biomed Res. Int. 2015, 498957.
  2. Kalbassi, S. et al. (2017) eNeuro 4, e0145.
Scientific background

Neuroligins (Nlgns) are a family of postsynaptic transmembrane cell adhesion proteins that play a crucial role in excitation and inhibition balance through their regulation of GABAergic and glutamatergic signaling. 5 Neuroligin family members have been identified in humans: NL1, NL2, NL3, NL4X, and NL4Y.  NL 1-3 have close homologs in mice. Neurogilins have been identified in rodents, chicken, Drosophila, and C. elegans. 

Neuroligin structure contains extracellular acetylcholinesterase like domain that lacks esterase activity, a single transmembrane domain, and a short cytoplasmic tail containing a type I PDZ-domain-binding motif that functions in intracellular protein-protein interactions and signaling processes. Neuroligins and neurexins are known to interact with each other1,2.

Neuroligin 3 is encoded by the Nlgn3 gene and seems to be ubiquitously expressed in the brain. Despite this fact, Neuroligin 3 functions in synaptic transmission appears to be region specific, affecting the somatosensory cortex and the hippocampus differently2.

In humans, a missense mutation in Nlgn3 (Nlgn3R451C) causing reduced synaptic expression of the protein is associated with non-syndromic autism spectrum disorders. In addition, Nlgn3 deletion shows impaired social interaction and vocal communication in rodent models.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 12/08/2021

Anti-Neuroligin 3 (extracellular) Antibody (#ANR-037) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, analysis. The antibody recognizes an extracellular epitope, and could potentially be used for detecting the protein in living cells. It has been designed to recognize NLGN3 from rat, mouse, and human samples.

For research purposes only, not for human use
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