Anti-Cannabinoid Receptor 1 (extracellular)-FITC Antibody

CB1, CB-R, CNR1, CANN6, Brain-type cannabinoid receptor, Central cannabinoid receptor
Cat #: ACR-001-F
Alternative Name CB1, CB-R, CNR1, CANN6, Brain-type cannabinoid receptor, Central cannabinoid receptor
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
      • Peptide NKSLSSFKENEENIQC, corresponding to amino acid residues 84-99 of rat CB1 receptor (Accession P20272). Extracellular, N-terminus.
    Accession (Uniprot) Number P20272
    Gene ID 25248
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human - identical; mouse – 15/16 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Label Fluorescein isothiocyanate (FITC).
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Standard quality control of each lot Western blot analysis (unlabeled antibody), and direct flow cytometry (labeled antibody).
    Applications: fc
    May also work in: ic, lci
      • Direct flow cytometry of live intact human THP-1 monocytic leukemia cells:
        ___ Cells.
        ___ Cells + rabbit IgG isotype control-FITC.
        ___ Cells + Anti-Cannabinoid Receptor 1 (extracellular)-FITC Antibody (#ACR-001-F), (2.5 µg).
        Direct flow cytometry of live intact mouse P815 mastocytoma cell line:
        ___ Cells.
        ___ Cells + rabbit IgG isotype control-FITC.
        ___ Cells + Anti-Cannabinoid Receptor 1 (extracellular)-FITC Antibody (#ACR-001-F), (5 µg).
      • Cannabinoids have been used in Eastern medicine for many years as pain relievers. Δ9-tetrahydrocannabinol (THC), the major psychoactive compound in marijuana and hashish, has been shown to interact with two specific cannabinoid receptors: cannabinoid receptor 1 (CB1 receptor) and cannabinoid receptor 2 (CB2 receptor).1 The cannabinoid receptors can be distinguished by their amino acid sequences, signaling mechanisms, and tissue distributions.1 Both receptors belong to the G-protein coupled receptor (GPCR) superfamily. CB1 was shown to modulate several Ca2+ and K+ ion channels.1,2

        CB1 is primarily expressed in the central nervous system. However, expression of CB1 is also detected in the peripheral terminals, in non-neuronal peripheral tissues such as uterus, testes, spleen, as well as in cells of the immune system.2,3

        CB1 is implicated in many cellular functions such as neurotransmitter release, pain relief, cancer, and obesity.4,5 Growth inhibition of tumor cells was demonstrated following mixed CB1/CB2 agonist treatment in both prostate and non-melanoma skin cancers.4,5 Through their interaction with CB1, cannabinoid compounds stimulate appetite for sweets and palatable foods in particular, making CB1 an attractive therapeutic target for the treatment of obesity and eating disorders.6

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 07/04/2019

    Anti-Cannabinoid Receptor 1 (extracellular) Antibody (#ACR-001) is a highly specific antibody directed against an epitope of the rat CB1 receptor. The antibody can be used in western blot, immunohistochemistry, and live cell imaging applications. It has been designed to recognize CB1 in human, mouse, and rat samples.

    Anti-Cannabinoid Receptor 1 (extracellular)-FITC Antibody (#ACR-001-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

    For research purposes only, not for human use
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