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Anti-Human Cannabinoid Receptor 2 (extracellular) Antibody

CB2, CNR2

Cat #: ACR-003
Alternative Name CB2, CNR2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h
Immunogen
  • Peptide (C)NGSKDGLDSNPMKD, corresponding to amino acid residues 11-24 of human CB2 receptor (Accession P34972). Extracellular, N-terminus.
Accession (Uniprot) Number P34972
Gene ID 1269
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Canis, bovine - 10/14 amino acid residues identical.
RRID AB_2039797.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity Not recommended to use with rat and mouse samples.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ifc, lci, wb
May also work in: ih*, ip*
Western blot
  • Western blot analysis of human HL-60 acute promyelocytic leukemia cell line lysate (lanes 1 and 2) and human MCF-7 breast adenocarcinoma cell line lysate (lanes 3 and 4): 
    Western blot analysis of human HL-60 acute promyelocytic leukemia cell line lysate (lanes 1 and 2) and human MCF-7 breast adenocarcinoma cell line lysate (lanes 3 and 4): 
    1,3. Anti-Human Cannabinoid Receptor 2 (extracellular) Antibody  (#ACR-003), (1:200).
    2,4. Anti-Human Cannabinoid Receptor 2 (extracellular) Antibody, preincubated with Human Cannabinoid Receptor 2 (extracellular) Blocking Peptide (#BLP-CR003).
Immunocytochemistry
  • Expression of CB2 receptor in human LNCaP
    Expression of CB2 receptor in human LNCaP
    Immunocytochemical staining of living human prostate carcinoma cells (LNCaP). A. Extracellular staining of cells with Anti-Human Cannabinoid Receptor 2 (extracellular) Antibody (#ACR-003), (1:50), followed by goat-anti-rabbit-AlexaFluor-488 secondary antibody. B. Nuclear staining of LNCaP cells with Hoechst 33342. C. Live intact LNCaP cells.
Indirect flow cytometry
  • Cell surface detection of CB2 receptor by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells:
    Cell surface detection of CB2 receptor by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-Human Cannabinoid Receptor 2 (extracellular) Antibody (#ACR-003), (2.5μg) + goat-anti-rabbit-FITC.
References
  1. Brooks, J.W. and Fraquhar-Smith, M.A. (2003) Br. J. Anaesth. 3, 175.
  2. Howlett, A.C. (2002) Prostaglandins Other Lipid Mediat. 68, 619.
  3. Guo, J. and Ikeda, S.R. (2004) Mol. Pharmacol. 65, 665.
  4. Wotherspoon, G. et al. (2005) Neuroscience 135, 235.
  5. Gong, J.P. et al. (2006) Brain Res. 1071, 10.
  6. Fernandez-Ruiz, J. et al. (2007) Trends Pharmacol. Sci. 28, 39.
  7. Sarfaraz, S. et al. (2005) Cancer Res. 65, 1635.
  8. Sanchez, C. et al. (2001) Cancer Res. 61, 5784.
  9. Alberich Jorda, M. et al. (2004) Blood 104, 526.
Scientific background

Cannabinoids have been used as pain relievers in Eastern medicine for many years.1 To date, two specific cannabinoid receptors have been identified: cannabinoid receptor 1 (CB1) and cannabinoid receptor 2 (CB2).2 The cannabinoid receptors can be distinguished by their amino acid sequence, signaling mechanisms and tissue distribution.2 Both receptors belong to the G-protein coupled receptor superfamily and are coupled to Gi/0 G protein.2,3

The CB2 is highly expressed in cells of the immune system such as macrophages, lymphocytes natural killer cells and mast cells but has also been shown to be expressed, by both, in situ-hybridization and in immunohistochemistry, in spleen, thymus, and pancreas.1,2,4 CB2 expression in brain is still much less characterized than that of CB1. Recently, it was demonstrated that CB2 is expressed in the brain and might have a role in controlling fundamental processes such as proliferation and survival of neural cells.5,6

Overexpression of CB2 was reported in several cancers such as prostate, glioma and acute myeloid leukemias.7-9 In human astrocytoma a direct relation between CB2 expression and tumor malignancy was demonstrated.8 Activation of CB2R in vivo by its agonist JWH-133, completely blocked cell growth.8 In C6 glioma, it was shown that activation of the CB2 by JWH-133 resulted in internalization of only the CB2 and not CB1 leading to apoptosis of the cells. This may well be a new approach for the treatment of glioma.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 30/09/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the human CB2 receptor. Anti-Human Cannabinoid Receptor 2 (extracellular) Antibody (#ACR-003) can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize CB2 from human samples. The antibody is not recommended for use on rat and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

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