Overview
- Peptide (C)EQLTKETEGGNHS, corresponding to amino acid residues 215-227 of rat CaV1.3 (Accession P27732). Extracellular, 2nd loop repeat I.
Cell surface detection of CaV1.3 by direct flow cytometry in live intact mouse J774 macrophage cell line:___ Cells.
___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
___ Cells + Anti-CaV1.3 (CACNA1D) (extracellular)-FITC Antibody (#ACC-311-F), (5µg).
Cell surface detection of CaV1.3 by direct flow cytometry in live intact human Jurkat T-cell leukemia cell line:___ Cells.
___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
___ Cells + Anti-CaV1.3 (CACNA1D) (extracellular)-FITC Antibody (#ACC-311-F), (5µg).
- Catterall, W.A. et al. (2003) Pharmacol. Rev. 55, 579.
- IUPHAR
- Mangoni, M.E. et al. (2003) Proc. Natl. Acad. Sci. U.S.A. 100, 5543.
- Zhang, Z. et al. (2005) Circulation 112, 1936.
All L-type calcium channels are encoded by one of the CaV1 channel genes. These channels play a major role as a Ca2+ entry pathway in skeletal cardiac and smooth muscles as well as in neurons, endocrine cells and possibly in non-excitable cells such as hematopoetic and epithelial cells. All CaV1 channels are influenced by dihydropyridines (DHP) and are also referred to as DHP receptors. While the CaV1.1 and CaV1.4 isoforms are expressed in restricted tissues (skeletal muscle and retina, respectively), the expression of CaV1.2 is ubiquitous.1,2
The CaV1.3 channels are also expressed, as are other L-type channels, in neurons and neuroendocrine cells. However, accumulated data has shown the expression of CaV1.3 in heart and suggests that it plays a major role in the generation of cardiac pacemaker activity.3,4
Several peptidyl toxins have been described that are specific L-type channel blockers. These include the Mamba toxins Calcicludine (#SPC-650), Calciseptine (#C-500) and FS-2 (#F-700). So far no selective blocker for one of the CaV1 isoforms has been described.
Anti-CaV1.3 (CACNA1D) (extracellular) Antibody (#ACC-311) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry and flow cytometry applications. It has been designed to recognize CaV1.3 from rat, mouse and human samples.
Anti-CaV1.3 (CACNA1D) (extracellular)-FITC Antibody (#ACC-311-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.
Powered by Bioz