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A Blocker of Neuronal L-Type CaV Channels

Cat #: SPC-650
Alternative Name CAC
Lyophilized Powder yes
  • Bioassay Tested
  • Origin Synthetic peptide
    MW: 6979 Da
    Purity: >98% (HPLC)
    Form Lyophilized powder.
    Effective concentration 500 nM.
    Modifications Disulfide bonds between Cys7-Cys57, Cys16-Cys40 and Cys32-Cys53.
    Molecular formula C321H476N86O78S6.
    CAS No.: 178037-96-2
    Activity Calcicludine is a potent and specific antagonist of neuronal L-type CaV channels1.
    1. Schweitz, H. et al. (1994) Proc. Natl. Acad. Sci. U.S.A. 91, 878.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to two weeks at 4°C or three months at -20°C.
    Our bioassay
    • Alomone Labs Calcicludine inhibits CaV1.2 channel currents heterologously expressed in Xenopus oocytes.
      Alomone Labs Calcicludine inhibits CaV1.2 channel currents heterologously expressed in Xenopus oocytes.
      A. Representative time course of Calcicludine (#SPC-650) inhibition of CaV1.2 channels current. Membrane potential was held at -100 mV, current was elicited by a 100 ms voltage ramp to +60 mV every 10 sec, and significantly inhibited by application of 500 nM Calcicludine (green). B. Superimposed traces of CaV1.2 current following application of control (black) and of 500 nM Calcicludine (green), taken from the recording in A.
    References - Scientific background
    1. Schweitz, H. et al. (1994) Proc. Natl. Acad. Sci. U.S.A. 91, 878.
    2. Stotz, S.C. et al. (2000) J. Membr. Biol174, 157.
    Scientific background

    Calcicludine is a natural peptide isolated from the Dendroaspis angusticeps green mamba venom by a modification to the procedure of Schweitz et al.1 and purified to homogenity.

    Calcicludine was shown to be a specific neuronal L-type Cachannel blocker and was shown to inhibit other neuronal HVA Cachannels (N- and P-type) as well.1 1 µM Calcicludine had no effect on skeletal muscle Cachannels (CaV1.1) as well as on T-type (CaV3) channels in heart and neurons.1 Calcicludine was tested to confirm its ability to block spontaneous or K+-induced contraction of cardiac cells.1 It blocked CaV1.2 cloned channels with an IC50 of 88 nM and had very little effect on CaV2 family channels.2 In addition, the block was irreversible upon wash and never complete but reached a maximum of 58%.

    Target L-type Ca2+ channels
    Peptide Content: 100%
    Last update: 29/08/2021

    Calcicludine (#SPC-650) is a highly pure, synthetic, and biologically active peptide toxin.

    For research purposes only, not for human use
    Shipping and Ordering information