- Schweitz, H. et al. (1994) Proc. Natl. Acad. Sci. U.S.A. 91, 878.
- Alomone Labs Calcicludine inhibits CaV1.2 channel currents heterologously expressed in Xenopus oocytes.A. Representative time course of Calcicludine (#SPC-650) inhibition of CaV1.2 channels current. Membrane potential was held at -100 mV, current was elicited by a 100 ms voltage ramp to +60 mV every 10 sec, and significantly inhibited by application of 500 nM Calcicludine (green). B. Superimposed traces of CaV1.2 current following application of control (black) and of 500 nM Calcicludine (green), taken from the recording in A.
Calcicludine is a natural peptide isolated from the Dendroaspis angusticeps green mamba venom by a modification to the procedure of Schweitz et al.1 and purified to homogenity.
Calcicludine was shown to be a specific neuronal L-type CaV channel blocker and was shown to inhibit other neuronal HVA CaV channels (N- and P-type) as well.1 1 µM Calcicludine had no effect on skeletal muscle CaV channels (CaV1.1) as well as on T-type (CaV3) channels in heart and neurons.1 Calcicludine was tested to confirm its ability to block spontaneous or K+-induced contraction of cardiac cells.1 It blocked CaV1.2 cloned channels with an IC50 of 88 nM and had very little effect on CaV2 family channels.2 In addition, the block was irreversible upon wash and never complete but reached a maximum of 58%.
Calcicludine (#SPC-650) is a highly pure, synthetic, and biologically active peptide toxin.