Anti-CLIC4 Antibody

Cl- intracellular channel 4, Intracellular chloride ion channel p64H1
Cat #: ACL-024
Alternative Name Cl- intracellular channel 4, Intracellular chloride ion channel p64H1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
      • Peptide (C)NGLKEEDKEPLIE, corresponding to amino acid residues 8-20 of human CLIC4 (Accession Q9Y696). Intracellular, N-terminus.
    Accession (Uniprot) Number Q9Y696
    Gene ID 25932
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse - identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, wb
    May also work in: ifc, ih, ip
      • Western blot analysis of human pancreatic carcinoma PANC-1 (lanes 1 and 4), mouse kidney (lanes 2 and 5) and rat kidney (lanes 3 and 6) lysates:
        1-3. Anti-CLIC4 Antibody (#ACL-024), (1:200).
        4-6. Anti-CLIC4 Antibody, preincubated with the control peptide antigen.
      • Expression of CLIC4 in human pancreatic carcinoma PANC-1 cells
        Immunocytochemical staining of fixed and permeabilized human pancreatic carcinoma PANC-1 cells. A. Cells were stained with Anti-CLIC4 Antibody (#ACL-024), (1:200), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Cell nuclei were visualized using Hoechst 33342 (blue). C. Live image of the cells.
      • Chloride intracellular channel (CLIC) is a family of proteins encoded by six different genes (CLIC1-6)1-3.

        The localization of the various CLIC channels remains elusive and controversial since they can be either found in a soluble form in the cytoplasm or as integral membrane proteins (in intracellular compartments such as the nuclear membrane or the endoplasmic reticulum for example, or associated with the plasma membrane) via their single transmembrane domain1.

        When membrane associated, CLIC channels indeed function as ion channels (enabling the passage of Cl- ions). Non-channel functions are also attributed to CLICs and include cell cycle regulation, cell differentiation, and apoptosis. These different roles depend on whether CLICs are membrane bound or cytosolic1. For instance, CLIC4 is involved in keratinocyte differentiation in a TGFb-dependent manner4,5, in such a way that TGFb stimulation induces the expression and nuclear localization of CLIC4 and its subsequent interaction with transcription factors4. CLIC4 is also required for growth arrest and apoptosis under stress6.

        CLIC4 distribution is rather ubiquitous but shows higher expression level in the skin, kidney, brain and testes7.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 04/06/2019

    Anti-CLIC4 Antibody (#ACL-024) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunocytochemistry applications. It has been designed to recognize CLIC4 from mouse, rat, and human samples.

    For research purposes only, not for human use