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Anti-CLIC5 Antibody

Cl- intracellular channel 5

Cat #: ACL-025
Alternative Name Cl- intracellular channel 5
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide (C)DTNTHGDEKGSQRK, corresponding to amino acid residues 161-174 of rat CLIC5 (Accession Q9EPT8). Intracellular, C-terminus.
Accession (Uniprot) Number Q9EPT8
Gene ID 94272
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical; human - 9/14 amino acid residues identical.
RRID AB_2340921.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, wb
May also work in: ifc*, ip*
Western blot
  • Western blot analysis of mouse brain (lanes 1 and 3) and rat lung (lanes 2 and 4) lysates:
    Western blot analysis of mouse brain (lanes 1 and 3) and rat lung (lanes 2 and 4) lysates:
    1,2. Anti-CLIC5 Antibody (#ACL-025), (1:200).
    3,4. Anti-CLIC5 Antibody, preincubated with CLIC5 Blocking Peptide (#BLP-CL025).
Immunohistochemistry
  • Mouse ileum sections (Yoshida, S. et al. (2016) Biomed. Res. 37, 127).
Immunocytochemistry
  • Rat cardiomyocytes (Ponnalagu, D. et al. (2016) Data Brief 7, 1038.).
References
  1. Jentsch, T.J. et al. (2002) Physiol. Rev. 82, 503.
  2. Gagnon, L.H. et al. (2006) J. Neurosci. 26, 10188.
  3. Wegner, B. et al. (2010) Am. J. Physiol. 298, F1492.
  4. Berryman, M. and Bretscher, A. (2001) Mol. Biol. Cell 11, 1509.
  5. Littler, D.R. et al. (2010) FEBS Letters 584, 2093.
  6. Debska, G. et al. (2001) Acta Biochim. Polonica 48, 137.
  7. Bradford, E.M. et al. (2010) Am. J. Physiol. 298, R1531.
Scientific background

Chloride (Cl-) channels are membrane, anion-selective protein pores, which diffusively transport negative ions across their electrochemical gradient. Cl- channels are subdivided into six unique families, and their regulation mechanisms range from voltage-dependency, through G-protein activation, to mechanosensitivity; they may reside in either the plasma membrane or the membrane of intracellular compartments or both1. They are detected in the kidney, placenta, intestines, brain and the inner-ear1-4

The vertebrate Cl- intracellular channel (CLIC) family is composed of 6 highly conserved members (CLIC1-6); each exists as a ~250 residue protein which can assume both a soluble fold and a membrane-integral form. The latter consists of a single trans-membrane domain and is subsequently oligomerized to construct a functioning channel1,5. Interestingly, channels composed of CLIC4 and CLIC5 have been confirmed to be equally permeable to K+ and Cl-5.

CLIC5, to which there are two isoforms  - CLIC5A and CLIC5B - encoded by the same gene5, has first been isolated from human placenta6, and was shown, together with CLIC4 (with which it shares 76% homology), to be enriched in the apical microvilli-containing part of the trophoblast epithelium, where, unlike CLIC4, it interacts with the actin cytoskeleton4. CLIC5 shares about 40% homology with CLIC1-4 and is distributed to a high degree in myocytes, cardiomyocytes and stereocillia of the inner-ear2,4. Mice mutated in the Clic5 gene lack coordination and gradually become deaf2, show resistance to obesity, experience gastric ulcers and the resultant hemorrhage, and have higher incidence of entering torpor7.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Anti-CLIC5 Antibody (#ACL-025) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry and immunohistochemistry applications. It has been designed to recognize CLIC5 from mouse, rat, and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Western blot citations
  1. Mouse heart lysate.
    Ponnalagu, D. et al. (2016) Data Brief 7, 1038.
Immunohistochemistry citations
  1. Mouse ileum sections.
    Yoshida, S. et al. (2016) Biomed. Res. 37, 127.
Immunocytochemistry citations
  1. Rat cardiomyocytes.
    Ponnalagu, D. et al. (2016) Data Brief 7, 1038.
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