Anti-CNGA4 Antibody

Cyclic nucleotide-gated cation channel alpha-4, CNG4, Cyclic nucleotide-gated olfactory channel subunit OCNC2, OCNCb
    Cat #: APC-074
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: m, r
    May also work in: h
    Immunogen
    Peptide (C) SQDGKVKTTESTPPAPTK, corresponding to amino acid residues 2-19 of rat CNGA4 (Accession Q64359). Intracellular, N-terminus.
    Accession (Uniprot) Number Q64359
    Gene ID 85258
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 15/18 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
    May also work in: ic, ifc, ip
    Western blot
    Western blot analysis of rat brain lysate:
    1. Anti-CNGA4 Antibody (#APC-074), (1:200).
    2. Anti-CNGA4 Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Expression of CNGA4 in mouse olfactory bulb
    Immunohistochemical staining of CNGA4 channel in mouse olfactory bulb using Anti-CNGA4 Antibody (#APC-074). A. CNGA4 staining (green) appears in glomeruli (horizontal arrows) and outside the glomeruli (vertical arrows). To define the location of glomeruli (G), the same section was stained with mouse anti-synaptophysin (red) and the nissl stain DAPI (blue). B. Pre-incubation of Anti-CNGA4 Antibody with the peptide antigen blocks the staining in the olfactory bulb.
    References
    1. Biel, M. (2009) J. Biol. Chem. 284, 9017.
    2. Kaupp, U.B. and Seifert, R. (2002) Physiol. Rev. 82, 769.
    3. Zheng, T. and Zagotta, W.N. (2004) Neuron 42, 411.
    4. Munger, S.D. et al. (2001) Science 294, 2172.
    5. Kelliher, K.R. et al. (2003) Proc. Natl. Acad. Sci. U.S.A. 100, 4299.
    6. Biel, M. and Michalakis, S. (2007) Mol. Neurobiol. 35, 266.
    Scientific background

    Cyclic Nucleotide-Gated (CNG) channels belong to the superfamily of voltage-gated ion channels. Although permeable to various ions such as the monovalent Na+ and K+ ions, and the divalent Ca2+ ion, they are gated by the intracellular binding of the cyclic nucleotides cAMP and cGMP and not by voltage per se (CNGs bind preferably to cGMP)1.

    Six channels form this subfamily: The A subunit (CNGA1-4) and the B subunit (CNGB1 and CNGB3). Functional entities are formed by the assembly of four subunits. Each subunit consists of six membrane spanning domains, where the fourth transmembrane domain is highly positively charged, a pore domain between transmembrane domains five and six, and intracellular N- and C-termini. The cyclic nucleotide binding domain is located in the C-terminal region and is responsible for the channel gating upon cyclic nucleotide binding1.

    These channels are highly expressed in retinal photoreceptors and olfactory neurons where their role has been extensively studied2. CNG channels have also been detected in brain, testis and kidney although their role in these tissues has yet to be unraveled2.   

    When heterologously expressed all subunits with exception of CNGA4, CNGB1 and CNGB3 can form functional homomeric channels. In olfactory sensory neurons, CNG channels which are formed by two CNGA2, one CNGA4 subunits and an isoform of CNGB1, are activated by the binding of cAMP, whose levels increase in response to the binding of odorant molecules to their respective receptors1,3.

    CNGA4 knockout mice are fertile and morphologically similar to their wild type littermates4,5. In these mice, the olfactory bulb and epithelium are developed but these mice display defects in odor adaptation, and at the molecular level, they are less sensitive to cAMP activation6.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 17/01/2019

    Anti-CNGA4 Antibody (#APC-074) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize CNGA4 from human, rat, and mouse samples.

    For research purposes only, not for human use