Anti-CRMP1 Antibody

CRMP-1, Collapsin response mediator protein 1, Dihydropyrimidinase-related protein 1, DRP-1, Dpysl1, Dihydropyrimidinase-like 1, Ulip3, Unc-33-like phosphoprotein 3
    Cat #: AIP-028
    Alternative Name CRMP-1, Collapsin response mediator protein 1, Dihydropyrimidinase-related protein 1, DRP-1, Dpysl1, Dihydropyrimidinase-like 1, Ulip3, Unc-33-like phosphoprotein 3
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)RVRIRSKVFGLHSVSR, corresponding to amino acid residues 481-496 of rat CRMP1 (Accession Q62950). Intracellular.
    • Anti-CRMP1 Antibody
    Accession (Uniprot) Number Q62950
    Gene ID 25415
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse – identical; human – 12/16 amino acid residues identical.
    RRID AB_2756658.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ifc*, ih*
    Western blot
    • Anti-CRMP1 Antibody
      Western blot analysis of mouse brain (lanes 1 and 5), rat cortex (lanes 2 and 6), rat new born brain (lanes 3 and 7) and human brain glioblastoma (U-87 MG) cell line lysates:
      1-4. Anti-CRMP1 Antibody (#AIP-028), (1:200).
      5-8. Anti-CRMP1 Antibody, preincubated with the negative control antigen.
    References
    1. Hou, H. et al. (2016) Oncol. Lett. 12, 23.
    2. Cai, G. et al. (2017) Oncogene 36, 546.
    3. Liu, S.H. et al (2015) Acta Crystallogr. F Struct. Biol. Commun. 71, 938.
    Scientific background

    Collapsin response mediator proteins (CRMPs) are a family of cytosolic phosphoproteins that were originally identified in developing nervous tissue. They are characterized as intracellular mediators of Semaphorin 3A signaling pathway, which is involved in axon differentiation during neural development.

    The CRMP family includes five members; CRMP-1 to CRMP-5. All five isoforms share a significant similarity in sequence with liver dihydropyrimidinase (DHPase) and with each other. CRMP-1 to CRMP-4 share 75% identical sequence, while CRMP-5 shares 50% identity with the other CRMPs.

    CRMP-1 is involved in cortical neuronal migration through reelin signaling. CRMP-1 inhibits the migration and invasion of non-small cell lung cancer (NSCLC). CRMP-1 includes a long form isoform, LCRMP-1, which promotes filopodia formation, cancer cell migration and invasion, resulting in poor clinical outcomes in NSCLC patients, therefore the long form is considered to be an invasion enhancer in NSCLC1-3.

    Human CRMP-1 overall structure is very similar to mouse CRMP-1. The human protein generally comprises a heterotetrameric assembly with a randomly coiled link that connects the protomers to each other. The proteins can form homotetramers when purified from bovine brain. CRMPs contains a positively charged C-terminal region that is highly susceptible to proteolysis2,3.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-CRMP1 Antibody (#AIP-028) is a highly selective antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize CRMP-1 from human, mouse, and rat samples.

    For research purposes only, not for human use
    Shipping and Ordering information