- Peptide CEVSVTPKTVTPAS, corresponding to amino acid residues 504-517 of rat CRMP2 (Accession P47942). Intracellular.
- Western blot analysis of rat brain membrane (lanes 1 and 5), mouse brain membrane (lanes 2 and 6), human CCF-STTGI brain astrocytoma (lanes 3 and 7) and rat PC-12 peochromocytoma (lanes 4 and 8) cell line lysates:1-3. Anti-CRMP2 Antibody (#AIP-029), (1:200).
4. Anti-CRMP2 Antibody (1:500).
5-8. Anti-CRMP2 Antibody, preincubated with CRMP2 Blocking Peptide (#BLP-IP029).
- Expression of CRMP2 in rat spinal cord.Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections sections with Anti-CRMP2 Antibody (#AIP-029), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. CRMP2 immunoreactivity (green) appears in the superficial layers of the dorsal horn (arrows). B. Pre-incubation of the antibody with CRMP2 Blocking Peptide (BLP-IP029), suppressed staining. Cell nuclei are stained with DAPI (blue)
Collapsin response mediator protein 2 (CRMP-2, DRP‐2), is a kinesin-binding protein and a member of the CRMP family, a novel family of microtubule-associated proteins (MAPs). This family consists of 5 highly conserved phosphorylated proteins: CRMP1-CRMP5. CRMP-2 was originally identified in the regulation of microtubule dimerization. CRMP-2 is the first member of the CRMP family identified in primary neuronal cells1,2.
Physiological functions of CRMP-2 protein include regulation of cell surface receptor endocytosis, kinesin-dependent axonal transport, growth cone collapse, polarity and differentiation of developing neurons and neurite outgrowth and the regulation of microtubule dynamics2.
Studies suggest that CRMP-2 can modulate neuronal viability through the PI3K/mTOR/S6K pathway. In addition, CRMP-2 expression has been detected in non-neuronal cells such as leukocytes, fibroblasts and neuroblastoma1.
Phosphorylation of CRMP-2 on specific Thr and Ser residues inactivates its activity, leading to inhibition of axonal growth and neuronal polarity. Knockdown of CRMP‐2 shortens the total dendritic length and decreases dendritic branching3.
Species reactivity key:
Anti-CRMP2 Antibody (#AIP-029) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize CRMP-2 from rat, mouse, and human samples.