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Rat and mouse brain, human brain astrocytoma (CCF-STTGI) cell line and rat peochromocytoma (PC-12) cell line (1:200-1:2000).Western blot analysis of rat brain membrane (lanes 1 and 5), mouse brain membrane (lanes 2 and 6), human CCF-STTGI (lanes 3 and 7) and rat PC-12 (lanes 4 and 8) cell line lysates:1-3. Anti-CRMP-2 Antibody (#AIP-029), (1:200).
4. Anti-CRMP-2 Antibody (1:500).
5-8. Anti-CRMP-2 Antibody, preincubated with the control peptide antigen.
Collapsin response mediator protein 2 (CRMP-2, DRP‐2), is a kinesin-binding protein and a member of the CRMP family, a novel family of microtubule-associated proteins (MAPs). This family consists of 5 highly conserved phosphorylated proteins: CRMP1-CRMP5. CRMP-2 was originally identified in the regulation of microtubule dimerization. CRMP-2 is the first member of the CRMP family identified in primary neuronal cells1,2.
Physiological functions of CRMP-2 protein include regulation of cell surface receptor endocytosis, kinesin-dependent axonal transport, growth cone collapse, polarity and differentiation of developing neurons and neurite outgrowth and the regulation of microtubule dynamics2.
Studies suggest that CRMP-2 can modulate neuronal viability through the PI3K/mTOR/S6K pathway. In addition, CRMP-2 expression has been detected in non-neuronal cells such as leukocytes, fibroblasts and neuroblastoma1.
Phosphorylation of CRMP-2 on specific Thr and Ser residues inactivates its activity, leading to inhibition of axonal growth and neuronal polarity. Knockdown of CRMP‐2 shortens the total dendritic length and decreases dendritic branching3.