Anti-CysLTR1 (extracellular) Antibody

CYSLT1, Cysteinyl leukotriene receptor 1, Cysteinyl leukotriene D4 receptor, LTD4 receptor, G-protein coupled receptor HG55, HMTMF81
    Cat #: ALR-003
    Alternative Name CYSLT1, Cysteinyl leukotriene receptor 1, Cysteinyl leukotriene D4 receptor, LTD4 receptor, G-protein coupled receptor HG55, HMTMF81
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h
    May also work in: m*, r*
    Immunogen
    • Peptide (C)HNETKP(S)DSVLRMQK, corresponding to amino acid residues 261-275 of human CysLTR1 (Accession Q9Y271). 3rd extracellular loop.
    • Anti-CysLTR1 (extracellular) Antibody
    Accession (Uniprot) Number Q9Y271
    Gene ID 10800
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human - 14/15; mouse - 12/15 amino acid residues identical; rat - 11/15 amino acid residues identical.
    RRID AB_10658486.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ifc, wb
    May also work in: ic*, ih*, ip*, lci*
    Western blot
    • Anti-CysLTR1 (extracellular) Antibody
      Western blot analysis of acute monocytic leukemia THP-1 (lanes 1 and 4), promyelocytic leukemia HL-60 (lanes 2 and 5) and acute T-cell leukemia Jurkat (lanes 3 and 6) human cell lysates:
      1,2,3. Anti-CysLTR1 (extracellular) Antibody (#ALR-003), (1:200).
      4,5,6. Anti-CysLTR1 (extracellular) Antibody, preincubated with the control peptide antigen.
    Indirect flow cytometry
    • Anti-CysLTR1 (extracellular) Antibody
      Cell surface detection of CysLTR1 in live intact THP-1 (acute monocytic leukemia) cells:
      ___ Cells + goat-anti-rabbit-Cy5.
      ___ Cells + Anti-CysLTR1 (extracellular) Antibody (#ALR-003), (1:50) + goat-anti-rabbit-Cy5.
    • The control antigen is not suitable for this application.
    References
    1. Rovati, G.E. and Capra, V. (2007) ScientificWorldJournal 7, 1375.
    2. Samuelson, B. (1983) Science 220, 568.
    3. Funk, C.D. (2001) Science 294, 1871.
    4. Singh, R.K. et al. (2010) Pharmacology 85, 336.
    5. Kanaoka, Y. and Boyce, J.A. (2004) J. Immunol. 173, 1503.
    6. Capra, V. et al. (2006) Med. Res. Rev. 27, 469.
    Scientific background

    LTC4, LTD4, and LTE4 are leukotrienes (cys-LTs) derived from arachidonic acid as a result of immune or inflammatory stimuli1-3.

    The above mentioned cys-LTs bind and activate two receptors belonging to the G-protein coupled receptor superfamily, CysLTR1 and CysLTR2 (also named cysLT1 and cysLT2 respectively). There is evidence though that other receptors are yet to be identified4. These two receptors couple to both pertussis toxin sensitive (Gi/o) and insensitive (Gq, which initiates Ca2+ influx) G-proteins for their signaling. The G-protein coupled to is probably determined by which ligand binds the receptor as all three cys-LTs can activate the two receptors albeit with different affinities5. In mouse, CysLTR1 and CysLTR2 are subject to alternative splicing. CysLTR1 receptor expresses greater affinity for LTD4 and binds equally to LTC4 and LTE4 whereas CysLTR2 has greater and equal affinity towards LTC4 and LTD4 over LTE45.

    Human CysLTR1 is highly expressed in spleen, peripheral blood, leukocytes. It is less strongly expressed in lung, small intestine, pancreas and hardly or not expressed in liver, colon, kidney, skeletal muscle, testis heart and brain4. On the other hand, CysLTR2 is highly expressed in spleen and leukocytes. Its expression in the heart, brain, spinal cord and adrenal gland differs it from that of CysLTR14.

    Cys-LTs and their receptors are involved in respiratory diseases like asthma and other conditions such as cancer cardiovascular, gastrointestinal, skin and immune disorders1,6.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-CysLTR1 (extracellular) Antibody (#ALR-003) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize CysLTR1 from human, mouse, and rat samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
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