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Anti-ENaC α (SCNN1A) (extracellular) Antibody

Amiloride-sensitive sodium channel subunit alpha, Alpha-NaCH, Epithelial sodium channel α subunit, Alpha-ENaC, Nonvoltage-gated sodium channel 1 subunit alpha, SCNEA

Cat #: ASC-030
Alternative Name Amiloride-sensitive sodium channel subunit alpha, Alpha-NaCH, Epithelial sodium channel α subunit, Alpha-ENaC, Nonvoltage-gated sodium channel 1 subunit alpha, SCNEA
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)EELDRITEQTLFD, corresponding to amino acid residues 173-185 of rat ENaCα (Accession P37089). Extracellular loop.
Accession (Uniprot) Number P37089
Gene ID 25122
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human - identical.
RRID AB_10917439.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.6 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ifc*, ip*, lci*
Western blot
  • Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) kidney lysates:
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) kidney lysates:
    1,2. Anti-ENaC α (SCNN1A) (extracellular) Antibody (#ASC-030), (1:200).
    3,4. Anti-ENaC α (SCNN1A) (extracellular) Antibody, preincubated with ENaC α/SCNN1A (extracellular) Blocking Peptide (#BLP-SC030).
Immunohistochemistry
  • Mouse kidney sections (Wang, L. et al. (2015) J. Am. Soc. Nephrol. 26, 2678.).
References
  1. Árnadóttir, J. and Chalfie, M. (2010) Annu. Rev. Biophys. 39, 111.
  2. Schild, L. (2010) Biochim. Biophys. Acta 1802, 1159.
  3. Soundararajan, R. et al. (2010) J. Biol. Chem. 285, 30363.
  4. Diakov, A. and Korbmacher, C. (2004) J. Biol. Chem. 279, 38134.
  5. Chang, S.S. et al. (1996) Nat. Genet. 12, 248.
Scientific background

The DEG/ENaC family consists of Na+ selective channels. They are characterized by 2 transmembrane domains with a large extracellular loop, important for channel activity and intracellular N-and C-termini which are important for channel gating and for interacting with regulatory proteins respectively1,2.

ENaC is localized to the apical side of polarized epithelial cells like those in the kidney, lung, and distal colon and is responsible for maintaining proper Na+ balance. This channel is formed by oligomerization of three subunits, α, β, and γ usually with the α2β1γ1 combination2,3. In addition, the α subunit which is essential for channel activity undergoes phosphorylation in its C-terminal by SGK1 (serum and glucocorticoid-induced kinase 1) which is known to activate the channel at the plasma membrane4.

Pseudohypoaldosteronism type 1 (PHA-1) has an early onset and is depicted by severe dehydration, hyponatremia and hyperkalemia5. One form of the pathology is characterized by mutations in α, β, and γ subunits5. The severity of the diseases depends on the loss of activity of the channel2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 24/01/2021

Anti-ENaC α (SCNN1A) (extracellular) Antibody (#ASC-030) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It recognizes an extracellular domain and can potentially detect the channel in living cells. It has been designed to recognize ENaCα from human, rat, and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Western blot citations
  1. Mouse kidney lysate.
    Wang, L. et al. (2015) J. Am. Soc. Nephrol. 26, 2678.
  2. Rat collecting duct lysate (1:200).
    Kemp, B.A. et al. (2014) Endocrinology 155, 2658.
Immunohistochemistry citations
  1. Mouse kidney sections.
    Wang, L. et al. (2015) J. Am. Soc. Nephrol. 26, 2678.
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