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Anti-ENaC β (SCNN1B) (extracellular) Antibody

Amiloride-sensitive sodium channel subunit beta, Beta-NaCH, Epithelial sodium channel β subunit, Beta-ENaC, Nonvoltage-gated sodium channel 1 subunit beta, SCNEB

Cat #: ASC-019
Alternative Name Amiloride-sensitive sodium channel subunit beta, Beta-NaCH, Epithelial sodium channel β subunit, Beta-ENaC, Nonvoltage-gated sodium channel 1 subunit beta, SCNEB
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide EFNYRTIEESPANNI(C), corresponding to amino acid residues 498-513 of rat ENaCβ (Accession P37090). Extracellular.
Accession (Uniprot) Number P37090
Gene ID 24767
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical; human - 14/15 amino acid residues identical.
RRID AB_2040190.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ifc*, ip*, lci*
Western blot
  • Western blot analysis of rat kidney (lanes 1 and 3) and lung (lanes 2 and 4) lysates:
    Western blot analysis of rat kidney (lanes 1 and 3) and lung (lanes 2 and 4) lysates:
    1,2. Anti-ENaC β (SCNN1B) (extracellular) Antibody (#ASC-019), (1:200).
    3,4. Anti-ENaC β (SCNN1B) (extracellular) Antibody, preincubated with ENaC β/SCNN1B (extracellular) Blocking Peptide (#BLP-SC019).
Immunohistochemistry
  • Mouse kidney (1:200) (Yang, S.S. et al. (2010) Endocrinology 151, 1829.).
References
  1. Kellenberger, S. and Schild, S. (2002) Physiol. Rev. 82, 735.
  2. Snyder, P.M. (2002) Endocrine Rev. 23, 258.
  3. Garty, H. and Palmer, L.G. (1997) Physiol. Rev. 77, 359.
Scientific background

The amiloride-sensitive epithelial Na+ channel (ENaC) family includes 4 members: ENaCα, β, γ and δ. The ENaC subunits have a conserved topology consisting of two membrane-spanning domains with intracellular N and C-termini and a large glycosylated extracellular region.

The functional ENaC channel is a heteromer with a presumed stoichiometry of α2βγ while the δ subunit can substitute for the α subunit in some tissues. Interestingly, neither the β nor the γ subunits are capable of producing any current when expressed alone in heterologous systems, while in these systems the simultaneous presence of all three ENaC subunits will produce Na+ currents that resemble the endogenous channel. 

The ENaC channel is located in the luminal (apical) plasma membrane of several epithelial tissues such as kidney, lung, salivary glands and skin where it enables entry of Na+ into the cell along its electrochemical gradient and thus has a central role in the maintenance of renal Na+ balance as well as liquid balance in the lung. The central role of ENaC in the regulation of Na+ homeostasis and hence blood pressure is underscored by the identification of two human diseases that arise from either gain- or loss-of-function mutations of the ENaC channel. Liddle’s syndrome is an inherited form of hypertension that stems from a dominant mutation of the ENaC channel (in either the β or γ subunits) that results in excessive activity of the channel and hence increased Na+ absorption. Conversely, pseudoaldosteronism type I (PHA) is a dysfunction characterized by hypotension due to poor Na+ absorption that is associated with loss-of-function mutations which may occur in each of the three ENaC subunits.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 24/01/2021

Anti-ENaC β (SCNN1B) (extracellular) Antibody (#ASC-019) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize ENaCβ from rat, human, and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Western blot citations
  1. Mouse kidney lysate.
    Susa, K. et al. (2014) Hum. Mol. Genet. 23, 5052.
  2. Mouse kidney lysate (1:200).
    Yang, S.S. et al. (2007) Cell Metabol. 5, 331.
Immunohistochemistry citations
  1. Mouse kidney sections (1:200).
    Yang, S.S. et al. (2010) Endocrinology 151, 1829.
  2. Rat trigeminal ganglion and incisor sections (1:200).
    Hitomi, Y. et al. (2009) Biomed. Res. 30, 113.
  3. Mouse kidney sections (1:200).
    Yang, S.S. et al. (2007) Cell Metabol. 5, 331.
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