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Anti-ENaC γ (SCNN1G) (extracellular) Antibody

Amiloride-sensitive sodium channel subunit gamma, Epithelial sodium channel γ subunit, Gamma-ENaC, Gamma-NaCH, Nonvoltage-gated sodium channel 1 subunit gamma, SCNEG

Cat #: ASC-011
Alternative Name Amiloride-sensitive sodium channel subunit gamma, Epithelial sodium channel γ subunit, Gamma-ENaC, Gamma-NaCH, Nonvoltage-gated sodium channel 1 subunit gamma, SCNEG
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: r
May also work in: h*
Immunogen
  • Peptide (C)YGVKESRKRREAGS, corresponding to amino acid residues 129-142 of rat ENaCγ (Accession P37091). Extracellular.
Accession (Uniprot) Number P37091
Gene ID 24768
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human - 13/17 amino acid residues identical.
RRID AB_2040192.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ifc*, ip*, lci*
Western blot
  • Western blot analysis of rat lung lysate:
    Western blot analysis of rat lung lysate:
    1. Anti-ENaC γ (SCNN1G) (extracellular) Antibody (#ASC-011), (1:200).
    2. Anti-ENaC γ (SCNN1G) (extracellular) Antibody, preincubated with ENaC gamma/SCNN1G (extracellular) Blocking Peptide (#BLP-SC011).
Immunohistochemistry
  • Guinea pig inner ear sections (1:300) (Mori, Y. et al. (2009) J. Physiol. Sci. 59, 355.).
References
  1. Kellenberger, S. and Schild, S. (2002) Physiol. Rev. 82, 735.
  2. Snyder, P.M. (2002) Endocrine Rev. 23, 258.
Scientific background

The amiloride-sensitive epithelial Na+ channel (ENaCs) family includes 4 members: ENaCα, β, γ and δ. The ENaC channel is located in the luminal (apical) plasma membrane of  several epithelial tissues such as kidney, lung, salivary glands and skin.  

The functional channel is believed to be a multimer including a α (or δ) and a β and γ subunits  with a likely stoichiometry of α2βγ.

ENaC channel enable entry of Na+ into the cell  along its electrochemical gradient and thus has a central role in the maintenance of  renal Na+ balance (and hence blood pressure) and liquid balance in the lung. Indeed, genetic mutations in the ENaC subunits causes Liddle’s syndrome (a form of hypertension) or pseudohypoaldosteronism type 1 (PHA) that is characterized by hypotension. 

The ENaC channel is voltage-independent and is constitutively active in epithelia although  it is modulated by several different mechanisms. One of the main mechanisms is the controlled internalization of the channel that is dependent  on the β or γ subunits. Indeed, mutations in the C-termini of these subunits reduce endocytosis  of the channel leading to the accumulation of ENaC in the cell membrane and causing a phenotype that is consistent with that of Liddle’s syndrome.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 24/01/2021

Anti-ENaC γ (SCNN1G) Antibody (#ASC-011) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular domain and can potentially detect the channel in living cells. It has been designed to recognize ENaCγ from rat and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Immunohistochemistry citations
  1. Guinea pig inner ear sections (1:300).
    Mori, Y. et al. (2009) J. Physiol. Sci. 59, 355.
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