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Anti-Ephrin-A2 (extracellular) Antibody

EPH-related receptor tyrosine kinase ligand 6, LERK6, CEK7-ligand, EFNA2
Cat #: AER-032
Alternative Name EPH-related receptor tyrosine kinase ligand 6, LERK6, CEK7-ligand, EFNA2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)HYGAPLPPAERMER, corresponding to amino acid residues 71-84 of mouse Ephrin-A2 (Accession P52801). Extracellular.
Accession (Uniprot) Number P52801
Gene ID 13637
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat – identical; human – 13/14 amino acid residues identical.
RRID AB_2756585.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ifc, ih, wb
May also work in: ic*, lci*
Western blot
  • Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
    Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
    1,2. Anti-Ephrin-A2 (extracellular) Antibody (#AER-032), (1:400).
    3,4. Anti-Ephrin-A2 (extracellular) Antibody, preincubated with Ephrin-A2 (extracellular) Blocking Peptide (#BLP-ER032).
Immunohistochemistry
  • Expression of Ephrin-A2 in mouse hippocampus
    Expression of Ephrin-A2 in mouse hippocampus
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Ephrin-A2 (extracellular) Antibody (#AER-032), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. Ephrin-A2 staining (green) in hippocampal dentate gyrus appears in neurons (vertical arrows) and astrocytes (horizontal arrows). Cell nuclei are stained with DAPI (blue).
Indirect flow cytometry
  • Cell surface detection of Ephrin-A2 in live intact human Jurkat T-cell leukemia cells:
    Cell surface detection of Ephrin-A2 in live intact human Jurkat T-cell leukemia cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-Ephrin-A2 (extracellular) Antibody (#AER-032), 5 µg + goat-anti-rabbit-FITC.
References
  1. Homman-Ludiye, J. et al. (2017) Sci. Rep. 7, 11813.
  2. Mukai, M. et al. (2017) BMC Cell Biol. 18, 28.
  3. Xu, Q. et al. (2000) Philos. Trans. R. Soc. Lond. B Biol. Sci. 355, 993.
Scientific background

Eph receptor tyrosine kinases family and their ligands, ephrins, are membrane proteins that primarily regulate cell to cell repulsion and cell adhesion and movement by modulating the organization of the actin cytoskeleton mainly through Rho family GTPases. Eph receptors and ephrin ligand signaling regulaes many aspects of cell interaction in the developing neocortex, including migration and axonal target recognition. Ephrin signaling plays an important role in guiding neurons and their projections during embryonic development1,2.

In mammals, the Eph receptor tyrosine kinases family has 14 members that are divided into 2 subclasses, the EphA (A1–A8 and A10) and EphB (B1–B4 and B6). This division is based on the sequence similarity of their extracellular domains. EphA receptors bind to glycosylphosphatidyl inositol-anchored ephrin-A ligands, and EphB receptors bind to transmembrane ephrin-B proteins.                                                              

The ephrin-A proteins include five members ephrin-A1 to ephrin-A5 whereas ephrin-B proteins include three ephrin-B1 to ephrin-B32,3.

Ephrin-A2 protein is abundantly expressed in retinal axons. Mutations in Ephrin-A2 disrupts topographic ordering of projections in the developing visual system. A knockout of ephrin-A2 in adult mice shows focal patches of disorganized neocortical laminar architecture, ranging in severity from reduced neuronal density to a complete lack of neurons1,2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Anti-Ephrin-A2 (extracellular) Antibody (#AER-032) is a highly specific antibody directed against an epitope of mouse EFNA2. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry. It has been designed to recognize EFNA2 from rat, mouse, and human samples.

For research purposes only, not for human use
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