Anti-Ephrin-A5 (extracellular) Antibody

EPH-related receptor tyrosine kinase ligand 7, LERK-7, EFNA5
  • New
Cat #: AER-035
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)DRVFDVNDKVENSLE, corresponding to amino acid residues 172-186 of human Ephrin-A5 (Accession P52803). Extracellular.
    Accession (Uniprot) Number P52803
    Gene ID 1946
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, rat – identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
    May also work in: ic, ifc, ip, lci
    Western blot
    Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysate:
    1,2. Anti-Ephrin-A5 (extracellular) Antibody (#AER-035), (1:200).
    3,4. Anti-Ephrin-A5 (extracellular) Antibody, preincubated with the control peptide antigen.
    Western blot analysis of human LNCaP prostate adenocarcinoma cell line lysate:
    1. Anti-Ephrin-A5 (extracellular) Antibody (#AER-035), (1:200).
    2. Anti-Ephrin-A5 (extracellular) Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Expression of Ephrin-A5 rat hippocampus
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Ephrin-A5 (extracellular) Antibody (#AER-035), (1:200), followed by goat anti-rabbit-AlexaFluor-488. Ephrin A5 staining (green) in hippocampal dentate gyrus is detected in hilar interneurons (arrows) and in the granule layer (GL). Cell nuclei are stained with DAPI (blue).
    References
    1. Liu, Y. et al. (2018) PLoS One 13, e0198291.
    2. Cheng, C. et al. (2017) Exp. Eye Res. 162, 9.
    3. Das, G. et al. (2016) Cell Biosci. 6, 48.
    Scientific background

    The Eph family of tyrosine kinase receptor signaling pathway is important for a variety of physiological processes during development including cell morphology, proliferation, migration and axonal guidance, dendritic spine formation and neural plasticity. Eph receptors mediate forward signaling in one cell while ephrin ligands transmit reverse signaling in an adjacent cell1-3.

    Eph receptors consist of two large subfamilies: EphA and EphB that bind to either glycophosphatidylinositol (GPI)-anchored ephrin-A ligands or transmembrane ephrin-B ligands respectively. Ephrin ligands are divided into ephrin-A (1 to 5) and ephrin-B (1 to 3)1,2.

    Ephrin-A5 is predominantly expressed in the quiescent anterior lens epithelial cells and is highly expressed in different layers of the cortex2,3.

    Studies show that mutations in ephrin-A5 are linked to congenital and age-related cataracts in humans and mice. Loss of ephrin-A5 causes abnormal localization of E-cadherin and β-catenin in anterior epithelial cells and epithelial-mesenchymal-transition2.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 30/12/2018

    Anti-Ephrin-A5 (extracellular) Antibody (#AER-035) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize EFNA5 from rat, mouse, and human samples.

    For research purposes only, not for human use