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Anti-EAAT1 (GLAST) (extracellular)-FITC Antibody

Excitatory amino acid transporter 1, Sodium-dependent glutamate/aspartate transporter 1, GLAST-1, SLC1A3
Cat #: AGC-021-F
Alternative Name Excitatory amino acid transporter 1, Sodium-dependent glutamate/aspartate transporter 1, GLAST-1, SLC1A3
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)KQFKTSYEKRSFK, corresponding to amino acids 188-200 of rat EAAT1 (Accession P24942). 2nd extracellular loop.
Accession (Uniprot) Number P24942
Gene ID 29483
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical; human 12/13 amino acid residues identical.
RRID AB_2756597.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Label Fluorescein isothiocyanate (FITC).
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #AGC-021), and direct flow cytometry (labeled antibody).
Applications: fc
May also work in: ic*, lci*
Direct flow cytometry
  • Cell surface detection of EAAT1 in live intact mouse BV-2 microglia cells:
    Cell surface detection of EAAT1 in live intact mouse BV-2 microglia cells:
    ___ Cells.
    ___ Cells + Rabbit IgG isotype control-FITC.
    ___ Cells + Anti-EAAT1 (GLAST) (extracellular)-FITC Antibody (#AGC-021-F), (5 µg).
  • Cell surface detection of EAAT1 in live intact human MEG-01 megakaryoblastic leukemia cells:
    Cell surface detection of EAAT1 in live intact human MEG-01 megakaryoblastic leukemia cells:
    ___ Cells.
    ___ Cells + Rabbit IgG isotype control-FITC.
    ___ Cells + Anti-EAAT1 (GLAST) (extracellular)-FITC Antibody (#AGC-021-F), (5 µg).
References
  1. Scopelliti, A.J. et al. (2013) J. Biol. Chem. 288, 8250.
  2. Benarroch, E.E. (2010) Neurology. 74, 259.
  3. Broer, S. and Palacin, M. (2011) Biochem. J. 436, 193.
Scientific background

Glutamate transporters play important roles in the termination of excitatory neurotransmission and in providing cells with glutamate for metabolic purposes.

SLC1A3 (EAAT1) belongs to The SLC1 protein family which contains a range of human and prokaryotic glutamate and aspartate transporters. EAAT1 transports the acidic amino acids glutamate and aspartate against a concentration gradient generating a net movement of substrate and charge across the membrane. Glutamate transport by EAAT1 is coupled to the co-transport of three Na+ ions and one H+ ion, and the counter transport of one K+ ion1.

EAAT1 is mainly expressed in astrocytes but can also be found in oligodendrocytes. EAAT1, together with EAAT2, are responsible for the bulk of glutamate uptake in the brain. They have several important roles, including the termination of the synaptic effects of glutamate, prevention of potentially toxic accumulation of extracellular glutamate, supply of glutamate to the astrocyte for synthesis of glutamine, which is involved in ammonia detoxification and in the glutamine-glutamate cycle and signaling of the metabolic needs of nearby neurons and activation of glycolysis in astrocytes2.

EAAT1 has been implicated in the pathogenesis of episodic ataxia, a rare neurological disorder characterized by neonatal muscle stiffness and an exaggerated startle reflex induced by noise or touch3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Anti-EAAT1 (GLAST) (extracellular) Antibody (#AGC-021) is a highly specific antibody directed against an epitope of rat Excitatory amino acid transporter 1. The antibody can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize EAAT1 from rat, human and mouse samples.

Anti-EAAT1 (GLAST) (extracellular)-FITC Antibody (#AGC-021-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as live cell direct flow cytometry.

For research purposes only, not for human use
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