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Anti-GABA(A) δ Receptor (extracellular) Antibody

GABRD, Gamma-aminobutyric acid receptor subunit delta, GABA(A) receptor subunit delta

Cat #: AGA-014
Alternative Name GABRD, Gamma-aminobutyric acid receptor subunit delta, GABA(A) receptor subunit delta
  • KO Validated
  • Lyophilized Powder yes
    Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    May also work in: h*
    Immunogen
    • Peptide (C)HHGARAMNDIGDYVGSN, corresponding to amino acid residues 19-35 of rat GABA(A) δ receptor (Accession P18506).
    Accession (Uniprot) Number P18506
    Gene ID 29689
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 14/17 amino acid residues identical.
    RRID AB_2340938.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Standard quality control of each lot Western blot analysis.
    Applications: if, ifc, ih, lci, wb
    May also work in: ic*, ip*
    Western blot
    • Western blot analysis of rat brain (lanes 1 and 4), rat cerebellum (lanes 2 and 5) and mouse brain (lanes 3 and 6):
      Western blot analysis of rat brain (lanes 1 and 4), rat cerebellum (lanes 2 and 5) and mouse brain (lanes 3 and 6):
      1-3. Anti-GABA(A) δ Receptor (extracellular) Antibody (#AGA-014), (1:200).
      4-6. Anti-GABA(A) δ Receptor (extracellular) Antibody, preincubated with GABA(A) δ Receptor (extracellular) Blocking Peptide (#BLP-GA014).
    Immunohistochemistry
    • Expression of GABA(A) δ receptor in rat cerebellum
      Expression of GABA(A) δ receptor in rat cerebellum
      Immunohistochemical staining of GABA(A) δ receptor in rat cerebellum using Anti-GABA(A) δ Receptor (extracellular) Antibody (#AGA-014). GABA(A) δ receptor (red) appears exclusively in the granule layer (G). Above and below the granule layer is the molecular layer (M). DAPI is used as the counterstain (blue).
    Indirect flow cytometry
    • Cell surface detection of GABA(A) δ Receptor by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells: 
      Cell surface detection of GABA(A) δ Receptor by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells: 
      ___ Cells.
      ___ Cells + goat-anti-rabbit-FITC.
      ___ Cells + Anti-GABA(A) δ Receptor (extracellular) Antibody(#AGA-014), (2.5μg) + goat-anti-rabbit-FITC .
    References
    1. Owens, D.F. and Kriegstein, A.R. (2002) Nat. Rev. Neurosci. 3, 715.
    2. Whiting, P.J. (1999) Neurochem. Inter. 34, 387.
    3. Mihic, S.J. and Harris, R.A. (1997) Alcohol Health Res. World 21, 127.
    4. Neelands, T.R. et al. (1999) J. Neurosci. 19, 7057.
    5. Olsen, R.W. and Tobbin, A.J. (1990) FASEBS J. 4, 1469.
    Scientific background

    GABA (γ-aminobutyric acid) is the major inhibitory neurotransmitter in the brain. Its production, release, reuptake, and metabolism all occur in the nervous system1.

    The GABA transmitter interacts with two major types of receptors: ionotropic GABAA receptors (GABAAR) and metabotropic receptors (GABABR). GABAARs belong to the ligand-gated ion channel superfamily2. GABA inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells3. Binding of GABA to its GABAA receptor results in conformational changes that open a Cl- channel, producing an increase in membrane conductance that results in inhibition of neural activity2.

    GABAARs are heteropentamers, in which all five subunits contribute to the pore formation. To date, eight subunit isoforms have been cloned: α, β, γ, δ, ε, π, θ, and ρ1. Six α subunit isoforms have been found to exist in mammals (α1-α6). In most cases, native GABAA receptors consist of 2α, 2β, and 1γ subunits. The α subunit is the most common and is expressed ubiquitously. It determines the affinity of GABAARs for allosteric ligands.

    Each subtype has a unique regional expression in the brain, and individual neurons often express multiple subtypes4. For example, the α4 subunit is detected in the hippocampus, cortex, olfactory bulb and in the basal forebrain5.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 12/08/2021

    Anti-GABA(A) δ Receptor (extracellular) Antibody (#AGA-014) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GABA(A) δ from human, rat, and mouse samples.

    For research purposes only, not for human use

    Applications

    Citations

    Citations
    KO validation citations
    1. Immunohistochemistry and Western blot of mouse brain. Tested in GABRD knockout mice.
      Rudolph, S. et al. (2020) Cell Reports. 33, 108338.
    Western blot citations
    1. Mouse brain lysate (1:200).
      Seo, S. and Leitch, B. (2015) Neuroscience 306, 28.
    Immunohistochemistry citations
    1. Mouse brain sections.
      Sun, M.Y. et al. (2018) J. Neurosci. 38, 8128.

    Specifications

    Scientific Background

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