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- Peptide (C)HHGARAMNDIGDYVGSN, corresponding to amino acid residues 19-35 of rat GABA(A) δ receptor (Accession P18506).
- Western blot analysis of rat brain (lanes 1 and 4), rat cerebellum (lanes 2 and 5) and mouse brain (lanes 3 and 6):1-3. Anti-GABA(A) δ Receptor (extracellular) Antibody (#AGA-014), (1:200).
4-6. Anti-GABA(A) δ Receptor (extracellular) Antibody, preincubated with the negative control antigen.
- Expression of GABA(A) δ receptor in rat cerebellumImmunohistochemical staining of GABA(A) δ receptor in rat cerebellum using Anti-GABA(A) δ Receptor (extracellular) Antibody (#AGA-014). GABA(A) δ receptor (red) appears exclusively in the granule layer (G). Above and below the granule layer is the molecular layer (M). DAPI is used as the counterstain (blue).
- 1. Owens, D.F. and Kriegstein, A.R. (2002) Nat. Rev. Neurosci. 3, 715.
- 2. Whiting, P.J. (1999) Neurochem. Inter. 34, 387.
- 3. Mihic, S.J. and Harris, R.A. (1997) Alcohol Health Res. World 21, 127.
- 4. Neelands, T.R. et al. (1999) J. Neurosci. 19, 7057.
- 5. Olsen, R.W. and Tobbin, A.J. (1990) FASEBS J. 4, 1469.
GABA (γ-aminobutyric acid) is the major inhibitory neurotransmitter in the brain. Its production, release, reuptake, and metabolism all occur in the nervous system1.
The GABA transmitter interacts with two major types of receptors: ionotropic GABAA receptors (GABAAR) and metabotropic receptors (GABABR). GABAARs belong to the ligand-gated ion channel superfamily2. GABA inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells3. Binding of GABA to its GABAA receptor results in conformational changes that open a Cl- channel, producing an increase in membrane conductance that results in inhibition of neural activity2.
GABAARs are heteropentamers, in which all five subunits contribute to the pore formation. To date, eight subunit isoforms have been cloned: α, β, γ, δ, ε, π, θ, and ρ1. Six α subunit isoforms have been found to exist in mammals (α1-α6). In most cases, native GABAA receptors consist of 2α, 2β, and 1γ subunits. The α subunit is the most common and is expressed ubiquitously. It determines the affinity of GABAARs for allosteric ligands.
Each subtype has a unique regional expression in the brain, and individual neurons often express multiple subtypes4. For example, the α4 subunit is detected in the hippocampus, cortex, olfactory bulb and in the basal forebrain5.
Species reactivity key:
Anti-GABA(A) δ Receptor (extracellular) Antibody (#AGA-014) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GABA(A) δ from human, rat, and mouse samples.
- Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001)
- Anti-GABA(A) α3 Receptor (extracellular) Antibody (#AGA-003)
- Anti-GABA(A) α4 Receptor (extracellular) Antibody (#AGA-008)
- Anti-GABA(A) α6 Receptor (extracellular) Antibody (#AGA-004)
- Anti-GABA(A) γ2 Receptor (extracellular) Antibody (#AGA-005)
- Anti-GABA(A) ρ1 Receptor (GABRR1) (extracellular) Antibody (#AGA-006)