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Anti-GABA(A) α6 Receptor (extracellular) Antibody

γ-Aminobutyric acid receptor type A subunit α6, GABRA6
Cat #: AGA-004
Alternative Name γ-Aminobutyric acid receptor type A subunit α6, GABRA6
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide QLEDEGNFYSENVSRILDN(C), corresponding to amino acid residues 20-37 of rat GABRA6 (Accession P30191). Extracellullar, N-terminus.
Accession (Uniprot) Number P30191
Gene ID 29708
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical; human - 17/19 amino acid residues identical.
RRID AB_2039868.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.7 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, lci, wb
May also work in: ifc*, ip*
Western blot
  • Western blot analysis of rat brain membranes:
    Western blot analysis of rat brain membranes:
    1. Anti-GABA(A) α6 Receptor (extracellular) Antibody, (1:200).
    2. Anti-GABA(A) α6 Receptor (extracellular) Antibody, preincubated with GABA(A) α6 Receptor (extracellular) Blocking Peptide (#BLP-GA004).
Immunohistochemistry
  • Expression of GABRA6 in mouse cerebellum
    Expression of GABRA6 in mouse cerebellum
    Immunohistochemical staining of mouse cerebellum using Anti-GABA(A) α6 Receptor (extracellular) Antibody (#AGA-004). A. GABRA6 (red) appears in the granule layer (asterisk). B. Parvalbumin (green) appears in Purkinje cells and in the molecular layer. C. Merge of GABRA6 and parvalbumin indicates that GABRA6 is restricted to granule cells. DAPI is used as the counterstain (blue).
Live cell imaging / Immunocytochemistry
  • Expression of GABRA6 in rat PC12 cells
    Expression of GABRA6 in rat PC12 cells
    Cell surface detection of GABRA6 in intact living rat pheochromocytoma (PC12) cells. A. Extracellular staining of cells using Anti-GABA(A) α6 Receptor (extracellular) Antibody (#AGA-004), (1:100), (red). B. Merge of A with the live view of the cell.
References
  1. Owens, D.F. and Kriegstein, A.R. (2002) Nature Rev. Neurosci. 3, 715.
  2. Whiting, P.J. (1999) Neurochem. Inter. 34, 387.
  3. Loh, E.W and Ball, D. (2000) Neurochem. Int. 37, 413.
  4. Surendran, S. et al. (2003) Mol. Gen. Met. 80, 74.
Scientific background

GABA (γ-aminobutyric acid) is the major inhibitory neurotransmitter in the brain. Its production, release, reuptake and metabolism occur in the nervous system.1

The GABA transmitter interacts with two major types of receptors: ionotropic GABA(A) and metabotropic GABA(B) receptors. The GABA(A) receptor belongs to the ligand gated ion channel superfamily.2

The GABA(A) R, is a heteropentamer, with all of its five subunits contributing to the pore formation.

To date, eight subunit isoforms were cloned: α, β, γ, δ, ε, π, θ, and ρ.1 Six α subunit isoforms were described to exist in mammals (α1-α6). The native GABA(A) receptor, in most cases, consists of 2α, 2β and 1γ subunit. The α subunit is the most common subunit and is expressed ubiquitously. It determines the affinities for allosteric ligands shown by GABA(A)R.

A possible role for the GABA(A) receptors in alcohol action has been derived from animal studies, in vitro cell models and human research which suggested an important role for GABA(A) α6 along with β2, α1 and γ2, in alcohol related phenotypes.3

In Canavan disease (inherited leukodystrophy), low level of expression of GABA(A) α6 was reported.4

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 16/08/2020

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the rat GABA(A) α6 receptor. Anti-GABA(A) α6 Receptor (extracellular) Antibody (#AGA-004) can be used in western blot, immunohistochemical and live cell imaging applications. It has been designed to recognize GABRA6 from rat, mouse and human samples.

For research purposes only, not for human use
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