Anti-GABA(A) α6 Receptor (extracellular) Antibody

Gamma-aminobutyric-acid receptor type A α6 subunit, GABRA6
    Cat #: AGA-004
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: m, r
    May also work in: h
    Immunogen
    Peptide QLEDEGNFYSENVSRILDN(C), corresponding to amino acid residues 20-37 of rat GABA(A) α6 Receptor (Accession P30191). Extracellullar, N-terminus.
    Accession (Uniprot) Number P30191
    Gene ID 29708
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 17/19 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.7 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ih, lci, wb
    May also work in: ifc, ip
    Western blot
    Western blot analysis of rat brain membranes:
    1. Anti-GABA(A) α6 Receptor (extracellular) Antibody, (1:200).
    2. Anti-GABA(A) α6 Receptor (extracellular) Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Expression of GABA(A) α6 Receptor in mouse cerebellum
    Immunohistochemical staining of mouse cerebellum using Anti-GABA(A) α6 Receptor (extracellular) Antibody (#AGA-004). A. GABA(A) α6 Receptor (red) appears in the granule layer (asterisk). B. Parvalbumin (green) appears in Purkinje cells and in the molecular layer. C. Merge of GABA(A) α6 Receptor and parvalbumin indicates that GABA(A) α6 Receptor is restricted to granule cells. DAPI is used as the counterstain (blue).
    Live cell imaging / Immunocytochemistry
    Expression of GABA(A) α6 receptor in rat PC12 cells
    Immunocytochemical staining of intact living rat pheochromocytoma (PC12) cells. A. Extracellular staining of cells using Anti-GABA(A) α6 Receptor (extracellular) Antibody (#AGA-004), (1:100), (red). B. Merge of A with the live view of the cell.
    References
    1. Owens, D.F. and Kriegstein, A.R. (2002) Nature Rev. Neurosci. 3, 715.
    2. Whiting, P.J. (1999) Neurochem. Inter. 34, 387.
    3. Loh, E.W and Ball, D. (2000) Neurochem. Int. 37, 413.
    4. Surendran, S. et al. (2003) Mol. Gen. Met. 80, 74.
    Scientific background

    GABA (γ-aminobutyric acid) is the major inhibitory neurotransmitter in the brain. Its production, release, reuptake and metabolism occur in the nervous system.1 

    The GABA transmitter interacts with two major types of receptors: ionotropic GABA(A) and the metabotropic GABA(B) receptors. The GABA(A) receptor belongs to the ligand gated ion channel superfamily.2 

    The GABA(A) R, is a heteropentamer, with all of its five subunits contributing to the pore formation. 

    To date, eight subunit isoforms were cloned: α, β, γ, δ, ε, π, θ, and ρ.1  Six α subunit isoforms were described to exist in mammals (α1-α6). The native GABA(A) receptor, in most cases, consists of 2α, 2β and 1γ subunit. The α subunit is the most common subunit and is expressed ubiquitously.  
    It determines the affinities for allosteric ligands shown by GABA(A)R. 

    A possible role for the GABA(A) receptors in alcohol action has been derived from animal studies,  in vitro cell models and human research which suggested an important role for GABA(A) α6 along with β2, α1 and γ2, in alcohol related phenotypes.3  

    In Canavan disease (inherited leukodystrophy), low level of expression of GABA(A) α6 was reported.4

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 07/11/2018

    Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat GABA(A) α6 Receptor. The Anti-GABA(A) α6 Receptor (extracellular) Antibody (#AGA-004) can be used in western blot analysis, immunohistochemical and live cell imaging applications. It has been designed to recognize GABA(A) α6 Receptor from rat, mouse and human samples.

    For research purposes only, not for human use