Anti-GABA(A) α4 Receptor (extracellular) Antibody

γ-Aminobutyric acid receptor subunit α4, GABRA4
    Cat #: AGA-008
    Alternative Name γ-Aminobutyric acid receptor subunit α4, GABRA4
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    • Peptide NSKDEKLSPENFTR(C), corresponding to amino acid residues 37-50 of rat GABRA4 with replacement of C44 with serine (Accession P28471). Extracellular, N-terminus.
    • Anti-GABA(A) α4 Receptor (extracellular) Antibody
    Accession (Uniprot) Number P28471
    Gene ID 140675
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, rat - 13/14 amino acid residues identical; human - 10/14 amino acid residues identical.
    RRID AB_10917596.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, lci, wb
    May also work in: ifc*, ip*
    Western blot
    • Anti-GABA(A) α4 Receptor (extracellular) Antibody
      Western blot analysis of rat brain lysate (lanes 1 and 4), mouse brain membrane (lanes 2 and 5) and CCF-STGI cell line lysate (lanes 3 and 6):
      1-3. Anti-GABA(A) α4 Receptor (extracellular) Antibody (#AGA-008), (1:200).
      4-6. Anti-GABA(A) α4 Receptor (extracellular) Antibody, preincubated with the negative control antigen.
    • Anti-GABA(A) α4 Receptor (extracellular) Antibody
      Expression of GABA(A) α4 Receptor in rat cerebellum
      Immunohistochemical staining of rat cerebellum using Anti-GABA(A) α4 Receptor (extracellular) Antibody (#AGA-008). A. GABRA4 staining (green) appears in the molecular layer (Mol) and around the soma of Purkinje cells (arrow). B. Parvalbumin (red), a marker of Purkinje and interneuronal cells, is stained in the same section. C. Merge of the images demonstrates expression of GABRA4 around the soma of Purkinje cells. DAPI is used as the counterstain (blue).
    Live cell imaging / Immunocytochemistry
    • Anti-GABA(A) α4 Receptor (extracellular) Antibody
      Expression of GABA(A) α4 Receptor in rat PC12 cells
      Cell surface detection of GABA(A) α4 Receptor in intact living rat pheochromocytoma PC12 cells. A. Extracellular staining of cells using Anti-GABA(A) α4 Receptor (extracellular) Antibody (#AGA-008), (1:50), (red). B. Merge of A with the live view of the cell.
    1. Owens, D.F. and Kriegstein, A.R. (2002) Nat. Rev. Neurosci. 3, 715.
    2. Whiting, P.J. (1999) Neurochem. Int. 34, 387.
    3. Mihic, S.J. and Harris, R.A. (1997) Alcohol Health Res. World 21, 127.
    4. Neelands, T.R. et al. (1999) J. Neurosci. 19, 7057.
    5. Olsen, R.W. and Tobbin, A.J. (1990) FASEB J. 4, 1469.
    Scientific background

    GABA (γ-aminobutyric acid) is the major inhibitory neurotransmitter in the brain. Its production, release, reuptake, and metabolism all occur in the nervous system1.

    The GABA transmitter interacts with two major types of receptors: ionotropic GABAA receptors (GABAAR) and metabotropic GABAB receptors (GABABR).

    GABAARs belong to the ligand-gated ion channel superfamily2. GABA inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells3. Binding of GABA to its GABAA receptor results in conformational changes that open a Cl- channel, producing an increase in membrane conductance that results in inhibition of neural activity2.

    GABAARs are heteropentamers, in which all five subunits contribute to the pore formation. To date, eight subunit isoforms have been cloned: α, β, γ, δ, ε, π, θ, and ρ1. Six α subunit isoforms have been found to exist in mammals (α1-α6). In most cases, native GABAA receptors consist of 2α, 2β, and 1γ subunits. The α subunit is the most common and is expressed ubiquitously. It determines the affinity of GABAARs for allosteric ligands.

    Each subtype has a unique regional expression in the brain, and individual neurons often express multiple subtypes4. For example, the α4 subunit is detected in the hippocampus, cortex, olfactory bulb and in the basal forebrain5.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 20/02/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat GABA(A) α4 subunit. Anti-GABA(A) α4 Receptor (extracellular) Antibody (#AGA-008) can be used in western blot, immunohistochemistry and live cell imaging applications. It was designed to recognize GABRA4 from human, rat and mouse samples.

    For research purposes only, not for human use
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