Anti-GFR alpha 4 (extracellular) Antibody

GDNF Family Receptor α4, Persephin Receptor, GFRα4, GFRA4
    Cat #: ANT-024
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: m, r
    Immunogen
    Peptide (C)GSRDG(S)PEEGGPR, corresponding to amino acid residues 160-172 of rat GFRα4 (Accession Q9EPI2). Extracellular, N-terminus.
    Accession (Uniprot) Number Q9EPI2
    Gene ID 66023
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat - 12/13 amino acid residues identical; mouse - 11/13 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Specificity Not recommended for human samples. The antibody will recognize both the membrane bound canonical isoform and the putative secreted isoform.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 2 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ih, lci, wb
    May also work in: ifc, ip
    Western blot
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:
    1-2. Anti-GFR alpha 4 (extracellular) Antibody (#ANT-024), (1:800).
    3-4. Anti-GFR alpha 4 (extracellular) Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Expression of GFRα4 in rat red nucleus
    Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-GFR alpha 4 (extracellular) Antibody (#ANT-024), (1:100). Staining reveals expression of GFRα4 (red) in cells with neuronal outline (arrows point at some examples) in the red nucleus. DAPI is used as the counterstain (blue).
    Live cell imaging / Immunocytochemistry
    Expression of GFRα4 in rat PC12 cells
    Immunocytochemical staining of live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells with Anti-GFR alpha 4 (extracellular) Antibody (#ANT-024), (1:100), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Cell nuclei stained with the cell permeable dye Hoechst 33342 (blue). C. Merge of the two images.
    References
    1. Airaksinen, M.S. and Saarma, M. (2002) Nat. Rev. Neurosci. 3, 383.
    2. Paratcha, G. and Ledola, F. (2008) Trends Neurosci. 31, 384.
    3. Paratcha, G. et al. (2001) Neuron 29, 171.
    Scientific background

    The GDNF family ligands (GFLs) belong to the super family of the TGF-β. They belong to the group of cystine-knot protein and function as homodimers1. This family includes glial cell line-derived neurotrophic factor (GDNF), artemin (ARTN), Neurturin (NRTN) and persephin (PSPN)2.

    These factors are heavily involved in the development and function of the nervous system (both central and peripheral). In particular GDNF has an important role outside the nervous system where it plays a role in kidney morphogenesis1.

    In general GFLs all signal through a signal through the receptor tyrosine kinase Ret. Their specificity is implemented by different GDNF family receptor a (GFRα), which act as co-receptors. These extracellular proteins are bound to the plasma membrane via a glycosyl phosphatidyl inositol (GPI) anchor. GFRα1-4 are responsible for the binding of GDNF, NRTN, ARTN, and PSPN respectively and the subsequent activation of Ret. Soluble forms of the receptor by the cleavage of a yet unknown phospholipase or protease can be detected1,3. Also alternative spliced forms of the protein can lead to soluble forms of GFRα receptors3.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 30/10/2018

    Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat GFRα4 receptor. Anti-GFR alpha 4 (extracellular) Antibody (#ANT-024) can be used in western blot analysis, immunohistochemical and immunocytochemical applications. Anti-GDNF Family Receptor α4 (extracellular) is highly suitable to be used in live cell imaging. It has been designed to recognize GFRα4 from mouse and rat samples. The antibody is not suitable for detecting GFRα4 from human samples.

    For research purposes only, not for human use