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Anti-GP130 (extracellular) Antibody

Interleukin-6 receptor subunit beta, IL-6R-beta, IL-6RB, Interleukin-6 signal transducer, Oncostatin-M receptor subunit alpha, CD130, Il6st
Cat #: ALR-023
Alternative Name Interleukin-6 receptor subunit beta, IL-6R-beta, IL-6RB, Interleukin-6 signal transducer, Oncostatin-M receptor subunit alpha, CD130, Il6st
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide (C)DTLYMVHMAAYTEEG, corresponding to amino acid residues 584-598 of rat GP130 (Accession P40190). Extracellular, N-terminus.
Accession (Uniprot) Number P40190
Gene ID 25205
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human - 13/15 amino acid residues identical.
RRID AB_2340989.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*, lci*
Western blot
  • Western blot analysis of mouse brain membrane (lanes 1 and 5), rat brain lysate (lanes 2 and 6), rat brain membrane (lanes 3 and 7) and rat uterus lysate (lanes 4 and 8):
    Western blot analysis of mouse brain membrane (lanes 1 and 5), rat brain lysate (lanes 2 and 6), rat brain membrane (lanes 3 and 7) and rat uterus lysate (lanes 4 and 8):
    1-3. Anti-GP130 (extracellular) Antibody (#ALR-023), (1:400).
    4. Anti-GP130 (extracellular) Antibody (1:200).
    5-8. Anti-GP130 (extracellular) Antibody, preincubated with GP130 (extracellular) Blocking Peptide (#BLP-LR023).
References
  1. Kurth, I. et al. (2000) J. Immunol. 164, 273.
  2. Kurth, I. et al. (1999) J. Immunol. 162, 1480.
  3. Timmermann, A. et al. (2002) Eur. J. Biochem. 269, 2716.
  4. Xu, S. et al. (2013) Expert Opin. Ther. Targets 17, 1303.
  5. Jones, S.A. et al. (2011) J. Clin. Invest. 121, 3375.
Scientific background

Interleukins (ILs) are key mediators in the regulation and coordination of the immune response. Glycoprotein 130 (gp130) belongs to the family of class I cytokine receptors and is the common signal transducing receptor subunit of the IL-6-type cytokines: IL-6, IL-11, leukemia inhibitory factor (LIF), ciliary neurotrophic factor (CNTF), oncostatin M (OSM), and cardiotrophin-11.The inflammatory cytokines IL-6 and IL-11 induce gp130 homodimerization after binding to their specific α receptors, which leads to the activation of the Janus kinase/STAT signal transduction pathway2. Gp130 Heterodimerizes with LIFR in response to LIF, CNTF, OSM, or CT13.

The extracellular part of gp130 contains an Ig-like domain (D1) followed by single cytokine binding module (CBM) (D2 and D3) and three fibronectin type III-like (FNIII) domains (D4, D5, and D6)3.

Gp130 mRNA is ubiquitously expressed in the human body, exhibiting highest levels in saphenous vein, pericardium, ovary, omental adipose tissue, peritoneum, spleen lymph nodes, and trigeminal ganglia4.

Dysregulation of gp130 expression, activation, or associated signaling pathways are implicated in a variety of human diseases including cancer, rheumatoid arthritis, Castleman’s disease, cardiac myxoma, Crohn’s disease, psoriasis, asthma, amyotrophic lateral sclerosis (CNTF), diabetes, atherosclerosis, systemic lupus, obesity, and postmenopausal osteoporosis5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 21/12/2020

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the rat Membrane glycoprotein 130. Anti-GP130 (extracellular) Antibody (#ALR-023) can be used in western blot analysis. It has been designed to recognize GP130 from mouse, rat and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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