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Anti-GPR174 (extracellular) Antibody

G-protein coupled receptor 174, LYPSR3, GPCR17, FKSG79
Cat #: AGR-060
Alternative Name G-protein coupled receptor 174, LYPSR3, GPCR17, FKSG79
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)DTSGNRTK(S)FVDLPTR, corresponding to amino acid residues 160 - 175 of human GPR174 (Accession Q9BXC1). 2nd extracellular loop.
Accession (Uniprot) Number Q9BXC1
Gene ID 84636
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human – 15/16 amino acid residues identical; mouse, rat – 13/16 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ifc, wb
May also work in: ic*, ih*, ip*, lci*
Western blot
  • Western blot analysis of rat brain membranes (lanes 1 and 5), mouse brain lysate (lanes 2 and 6), rat spleen lysate (lanes 3 and 7) and mouse spleen membranes (lanes 4 and 8):
    Western blot analysis of rat brain membranes (lanes 1 and 5), mouse brain lysate (lanes 2 and 6), rat spleen lysate (lanes 3 and 7) and mouse spleen membranes (lanes 4 and 8):
    1-4. Anti-GPR174 (extracellular) Antibody (#AGR-060), (1:400).
    5-8. Anti-GPR174 (extracellular) Antibody, preincubated with GPR174 (extracellular) Blocking Peptide (#BLP-GR060).
  • Western blot analysis of human MOLT-4 T-cell leukemia cell line lysate (lanes 1 and 3) and human Jurkat T-cell leukemia cell line lysate (lanes 2 and 4):
    Western blot analysis of human MOLT-4 T-cell leukemia cell line lysate (lanes 1 and 3) and human Jurkat T-cell leukemia cell line lysate (lanes 2 and 4):
    1, 2. Anti-GPR174 (extracellular) Antibody (#AGR-060), (1:400).
    3, 4. Anti-GPR174 (extracellular) Antibody, preincubated with GPR174 (extracellular) Blocking Peptide (#BLP-GR060).
Indirect flow cytometry
  • Cell surface detection of GPR174 in live intact human Jurkat T-cell leukemia cells:
    Cell surface detection of GPR174 in live intact human Jurkat T-cell leukemia cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-GPR174 (extracellular) Antibody (#AGR-060), (5 µg) + goat-anti-rabbit-FITC.
References
  1. Kihara, Y. et al. (2014) Br. J. Pharmacol. 171, 3575.
  2. Sugita, K. et al. (2013) Biochem. Biophys. Res. Commun. 430, 190.
  3. Zhao, S.X. et al. (2013) Hum. Mol. Genet. 22, 3347.
  4. Barnes, M.J. and Cyster, J.G. (2018) Immunol. Cell Biol. 96, 439.
Scientific background

GPR174 (LysoPS, LYPSR3), is one of three lysophosphatidyl serine receptors, belonging to the superfamily of G-protein coupled receptors. Lysophospholipids are a diverse group of small, membrane-derived phospholipids that act as extracellular signals1.

Activation of GPR174 leads to an increase in cAMP cellular levels, leading to cellular morphological changes, cell to cell adhesion, and delays in cell proliferation2.

GPR174 is reported to be a genetic risk locus for Graves disease, an autoimmune disorder that causes hyperthyroidism3 and as a negative regulator of T-cells activation4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

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Last update: 01/10/2020

Anti-GPR174 (extracellular) Antibody (#AGR-060) is a highly specific antibody directed against an epitope of the human receptor. The antibody can be used in western blot and indirect flow cytometry applications. It has been designed to recognize GPR174 from human, mouse, and rat samples.

For research purposes only, not for human use
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