Anti-Adenosine A2A Receptor (extracellular)-FITC Antibody

ADORA2A, A2AAR, Adenosine A2aR
  • New
Cat #: AAR-008-F
Alternative Name ADORA2A, A2AAR, Adenosine A2aR
  • Lyophilized Powder
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    Immunogen
    • Peptide (C)SQKDENSTKT(S)GE, corresponding to amino acid residues 143 - 156 of mouse A2A Adenosine Receptor (Accession Q60613). 2nd extracellular loop.
    • Anti-Adenosine A2A Receptor (extracellular)-FITC Antibody
    Accession (Uniprot) Number Q60613
    Gene ID 11540
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse – 13/14 amino acid residues identical; rat – 12/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG
    Specificity It will not recognize A2A Adenosine Receptor from human samples.
    Label Fluorescein isothiocyanate (FITC)
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #AAR-008), and direct flow cytometry (labeled antibody).
    Applications: fc
    May also work in: ic*, lci*
    Direct flow cytometry
    • Anti-Adenosine A2A Receptor (extracellular)-FITC Antibody
      Cell surface detection of Adenosine A2A Receptor by direct flow cytometry in live intact mouse J774 macrophage cells:
      ___ Cells.
      ___ Cells + rabbit IgG isotype control-FITC.
      ___ Cells + Anti-Adenosine A2A Receptor (extracellular)-FITC Antibody (#AAR-008-F), (2.5 µg).
    • Anti-Adenosine A2A Receptor (extracellular)-FITC Antibody
      Cell surface detection of Adenosine A2A Receptor by direct flow cytometry in live intact rat RBL-2H3 basophilic leukemia cells:
      ___ Cells.
      ___ Cells + rabbit IgG isotype control-FITC.
      ___ Cells + Anti-Adenosine A2A Receptor (extracellular)-FITC Antibody (#AAR-008-F), (2.5 µg).
    References
    1. Okusa, M.D. (2002) Am. J. Physiol. 282, F10.
    2. Fredholm, B.B. et al. (2001) Pharmacol. Rev53, 527.
    3. Nakata, H. (1989) J. Biol. Chem. 264, 16545.
    4. Cunha, R.A. (2001) Neurochem. Int. 38, 107.
    5. Fredholm, B.B. et al. (2000) Naunyn-Schmiedebergs Arch. Pharmacol362, 364.
    6. Canals, M. et al. (2005) J. Neurochem92, 337.
    7. Ohta, A. and Sitkovsky, M. (2001) Nature 414, 916.
    8. Ohta, A. et al. (2006) Proc. Natl. Acad. Sci. 103, 13132.
    Scientific background

    Adenosine is an endogenous nucleoside generated locally in tissues under conditions of hypoxia, ischemia, or inflammation. It modulates a variety of physiological functions in many tissues including the brain and heart1,2. Adenosine exerts its actions via four specific adenosine receptors (also named P1 purinergic receptors): Adenosine A1 Receptor (A1AR), Adenosine A2A Receptor (A2AAR), Adenosine A2B Receptor (A2BAR), and Adenosine A3 Receptor (A3AR). All are integral membrane proteins and are members of the G protein-coupled receptor superfamily. They share a common structure of seven putative transmembrane domains, an extracellular -NH2 terminus, cytoplasmic -COOH terminus, and a third intracellular loop important for binding G proteins.1-3 The adenosine receptors can be distinguished on the basis of their differential selectivity for adenosine analogs1-3.

    Adenosine receptors control neurotransmitter release through the facilitatory A2AAR and the inhibitory A1AR.4 A2AAR and A1AR are the major adenosine receptor subtypes expressed in the central nervous system (CNS). A2AAR is mainly expressed in the striatum on GABAergic striatopallidal neurons, while A1AR is widely distributed throughout the CNS5,6.

    A2AAR was suggested to play a critical role in attenuation of systemic inflammatory responses and prevention of extensive tissue damage.7 It was suggested that extracellular adenosine that accumulates in inflamed and damaged tissue may activate the A2AAR expressed in immune cells leading to termination/inhibition of the immune response.7 It was further suggested that this same mechanism may protect tumors from antitumor T cells through an immunosuppressive signal generated by the activation of A2AAR on T cells by extracellular adenosine produced from hypoxic cancerous tissues8.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 31/07/2020

    Anti-Adenosine A2A Receptor (extracellular) Antibody (#AAR-008) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize A2aR from mouse and rat samples. The antibody will not recognize the receptor from human samples.

    Anti-Adenosine A2A Receptor (extracellular)-FITC Antibody (#AAR-008-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
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