Anti-Human Adenosine A2A Receptor (extracellular) Antibody

Adenosine receptor A2A, A2AR, ADORA2A
  • New
Cat #: AAR-007
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h
    Immunogen
    Peptide CGQPKEGKNHSQG, corresponding to amino acid residues 146 - 158 of human adenosine A2A receptor (Accession P29274). 2nd extracellular loop.
    Accession (Uniprot) Number P29274
    Gene ID 135
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human only.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Specificity Will not recognize rat or mouse samples.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ifc, wb
    May also work in: ic, ih, ip, lci
    Western blot
    Western blot analysis of human HL-60 promyelocytic leukemia cell line lysate (lanes 1 and 3) and human Jurkat T-cell leukemia cell line lysate (lanes 2 and 4):
    1,2. Anti-Human Adenosine A2A Receptor (extracellular) Antibody (#AAR-007), (1:200).
    3,4. Anti-Human Adenosine A2A Receptor (extracellular) Antibody, preincubated with the control peptide antigen.
    Indirect flow cytometry
    Indirect flow cytometry of live intact human THP-1 monocytic leukemia cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-Human Adenosine A2A Receptor (extracellular) Antibody (#AAR-007), (2.5 µg) + goat-anti-rabbit-FITC.
    Indirect flow cytometry of live intact human Jurkat T-cell leukemia cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-Human Adenosine A2A Receptor (extracellular) Antibody (#AAR-007), (2.5 µg) + goat-anti-rabbit-FITC.
    References
    1. Okusa, M.D. (2002) Am. J. Physiol. 282, F10.
    2. Fredholm, B.B. et al. (2001) Pharmacol. Rev. 53, 527.
    3. Nakata, H. (1989) J. Biol. Chem. 264, 16545.
    4. Cunha, R.A. (2001) Neurochem. Int. 38, 107.
    5. Fredholm, B.B. et al. (2000) Naunyn-Schmiedebergs Arch. Pharmacol. 362, 364.
    6. Canals, M. et al. (2005) J. Neurochem. 92, 337.
    7. Ohta, A. and Sitkovsky, M. (2001) Nature 414, 916.
    8. Ohta, A. et al. (2006) Proc. Natl. Acad. Sci. 103, 13132.
    Scientific background

    Adenosine is an endogenous nucleoside generated locally in tissues under conditions of hypoxia, ischemia, or inflammation. It modulates a variety of physiological functions in many tissues including the brain and heart1,2. Adenosine exerts its actions via four specific adenosine receptors (also named P1 purinergic receptors): Adenosine A1 Receptor (A1AR), Adenosine A2A Receptor (A2AAR), Adenosine A2B Receptor (A2BAR), and Adenosine A3 Receptor (A3AR). All are integral membrane proteins and are members of the G protein-coupled receptor superfamily. They share a common structure of seven putative transmembrane domains, an extracellular -NH2 terminus, cytoplasmic -COOH terminus, and a third intracellular loop important for binding G proteins.1-3 The adenosine receptors can be distinguished on the basis of their differential selectivity for adenosine analogs1-3.

    Adenosine receptors control neurotransmitter release through the facilitatory A2AAR and the inhibitory A1AR.4 A2AAR and A1AR are the major adenosine receptor subtypes expressed in the central nervous system (CNS). A2AAR is mainly expressed in the striatum on GABAergic striatopallidal neurons, while A1AR is widely distributed throughout the CNS5,6.

    A2AAR was suggested to play a critical role in attenuation of systemic inflammatory responses and prevention of extensive tissue damage.7 It was suggested that extracellular adenosine that accumulates in inflamed and damaged tissue may activate the A2AAR expressed in immune cells leading to termination/inhibition of the immune response.7 It was further suggested that this same mechanism may protect tumors from antitumor T cells through an immunosuppressive signal generated by the activation of A2AAR on T cells by extracellular adenosine produced from hypoxic cancerous tissues8.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 24/03/2019

    Anti-Human Adenosine A2A Receptor (extracellular) Antibody (#AAR-007) is a highly specific antibody directed against an epitope of human protein. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize A2AR from human samples only. The antibody will not recognize the receptor from mouse or rat samples.

    For research purposes only, not for human use
    Citations
    Related Products
      • Products for control experiments
        1. Anti-Adenosine A2A Receptor Antibody (#AAR-002) . This antibody recognizes a different epitope and thus can also be used as a control for detecting Adenosine A2A Receptor.