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Anti-KCNS3 (KV9.3) (extracellular) Antibody

Potassium voltage-gated channel subfamily S member 3

Cat #: APC-133
Alternative Name Potassium voltage-gated channel subfamily S member 3
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)EFQNEDGEVDDPVLE, corresponding to amino acid residues 209-223 of rat KCNS3 (Accession O88759). 1st extracellular loop.
Accession (Uniprot) Number O88759
Gene ID 83588
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human - identical.
RRID AB_2341034.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*, lci*
Western blot
  • Western blot analysis of rat lung membranes (lanes 1 and 3) and mouse heart membranes (lanes 2 and 4):
    Western blot analysis of rat lung membranes (lanes 1 and 3) and mouse heart membranes (lanes 2 and 4):
    1,2. Anti-KCNS3 (KV9.3) (extracellular) Antibody (#APC-133), (1:200).
    3,4. Anti-KCNS3 (KV9.3) (extracellular) Antibody, preincubated with KCNS3/Kv9.3 (extracellular) Blocking Peptide (#BLP-PC133).
Immunohistochemistry
  • Expression of KV9.3 channel in rat cerebellum
    Expression of KV9.3 channel in rat cerebellum
    Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-KCNS3 (KV9.3) (extracellular) Antibody (#APC-133), (1:200). A. KV9.3 staining (red) is expressed in the molecular layer (M). B. DAPI counterstain (blue) displays the layout of cerebellar layers. C. Merge of A and B demonstrates restriction of KV9.3 expression to the molecular layer.
References
  1. Shepard, A.R. and Rae, J.L. (1999) Am. J. Physiol. 277, 412.
  2. Richardson, F.C. and Kaczmarek, L.K. (2000) Hear. Res. 147, 21.
  3. Costigan, M. et al. (2010) Brain 133, 2519.
Scientific background

K+ channels are transmembrane proteins expressed in many excitable and non-excitable cells. Functional entities are formed by the tetrameric assembly of α subunits which could be done in a homomeric or heteromeric fashion. In addition, the association of β subunits is also required for the proper function of K+ channels. Various splice variants are also expressed, thereby complicating the picture1,2.

K+ channels belonging to the KV9 subfamily resemble the delayed-rectifier class of K+ channel α subunits. These channels include six transmembrane domains, an ion selective pore, a leucine zipper and positively charged amino acids in S4 the voltage sensor domain. Interestingly, both KV9.1 and KV9.3 channels are electrically silent delayed rectifying K+ channels. However, they are responsible for modifying the activity of other K+ channels such as that of KV2.1, yielding currents different from those of KV2.1 on its own1.

KV9.1 is mainly expressed in the brain, human lens epithelial cells, kidney, prostate and testis. That of KV9.3 is more generalized and ubiquitous1.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 12/08/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the rat KV9.3 channel. Anti-KCNS3 (KV9.3) (extracellular) Antibody (#APC-133) can be used in western blot and immunohistochemistry applications. It has been designed to recognize KV9.3 from human mouse and rat samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

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