Anti-KV2.2 (KCNB2) Antibody

Voltage-gated potassium channel subfamily B member 2, CDRK
    Cat #: APC-120
    Alternative Name Voltage-gated potassium channel subfamily B member 2, CDRK
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide CRQDIYQAVGEVKKD, corresponding to amino acid residues 859-873 of rat KV2.2 (Accession Q63099). Intracellular, C-terminus.
        Anti-KV2.2 (KCNB2) Antibody
    Accession (Uniprot) Number Q63099
    Gene ID 117105
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 13/15 amino acid residues identical.
    RRID AB_2040164.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, ip, wb
    May also work in: ifc*
      • Anti-KV2.2 (KCNB2) Antibody
        Western blot analysis of rat brain lysate:
        1. Anti-KV2.2 (KCNB2) Antibody (#APC-120), (1:2000).
        2. Anti-KV2.2 (KCNB2) Antibody, preincubated with the control peptide antigen.
      • Rat brain membranes (Kihira, Y. et al. (2010) J. Biol. Chem. 285, 15048.).
      • Rat brain sections (Kihira, Y. et al. (2010) J. Biol. Chem. 285, 15048.).
      • HEK-293 cells transfected with rabbit KV2.2 (Kyle, B. et al. (2011) Am. J. Physiol. 301, C1186.).
      • KV2.2 is a member of the voltage-gated K+ channel superfamily. Together with the closely related KV2.1 protein they form the KV2 subfamily also known as Shab.

        As with all KV channels, KV2.2 possesses the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N and C termini. The functional KV channel is a tetramer that can either be a homotetramer or a heteromer of KV2.2 and KV2.1 subunits.

        Both KV2.2 and KV2.1 channels are known as delayed rectifiers that is, channels that are activated by changes in membrane potential (depolarization) but inactivate very slowly. The current they form is known as IK or IDR. Accessory subunits such as KChaP and the electrically silent a subunits KV8 and KV9 can modulate biochemical and biophysical properties of KV2.2. 

        KV2.2 is expressed in the brain and in peripheral tissues such as gastrointestinal and vascular smooth muscle. Physiologically, KV2.2 has a role in maintaining membrane potential and in modulating electrical excitability in neurons. In smooth muscle cells, KV2.2 is probably involved in maintaining the contractile capacity of the cells.

        The pharmacology of the KV2.2 channel is somewhat limited. The channel is blocked by the classical K+ channel blockers tetraethylammonium (TEA) and 4-aminopyridine (4-AP). More recently, the small peptide toxin Stromatoxin-1 has been found to be a potent and more specific inhibitor of the KV2 subfamily.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the rat KV2.2 channel. Anti-KV2.2 (KCNB2) Antibody (#APC-120) can be used in western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize KV2.2 from rat, human, and mouse samples.

    For research purposes only, not for human use
      • Rat 832/13 cells expressing human KV2.2 (1:1000).
        Jensen, M.V. et al. (2013) J. Biol. Chem. 288, 23128.
      • Rat 832/13 cells expressing human KV2.2 (2 µg / 5x106 cells).
        Jensen, M.V. et al. (2013) J. Biol. Chem. 288, 23128.
      • Rat brain membranes.
        Kihira, Y. et al. (2010) J. Biol. Chem. 285, 15048.
      • Rat lumbar spinal cord sections.
        Wolff, M. et al. (2016) Neurosci. Res. 109, 16.
      • Mouse brain sections (1:500).
        Blosa, M. et al. (2015) J. Physiol. 593, 4341.
      • Rat brain sections.
        Kihira, Y. et al. (2010) J. Biol. Chem. 285, 15048.
      • HEK-293 cells transfected with rabbit KV2.2.
        Kyle, B. et al. (2011) Am. J. Physiol. 301, C1186.
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