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Anti-LETM1 Antibody

Leucine zipper-EF-hand-containing transmembrane protein 1, LETM1 and EF-hand domain-containing protein 1 mitochondrial, Mitochondrial proton/calcium exchanger protein, Mdm38 homolog

Cat #: ANT-183
Alternative Name Leucine zipper-EF-hand-containing transmembrane protein 1, LETM1 and EF-hand domain-containing protein 1 mitochondrial, Mitochondrial proton/calcium exchanger protein, Mdm38 homolog
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)KELSKTGDEKYIEE, corresponding to amino acid residues 587-600 of rat LETM1 (Accession Q5XIN6). Intracellular, mitochondrial matrix.
Accession (Uniprot) Number Q5XIN6
Gene ID 305457
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse –13/14 amino acid residues identical; human – 12/14 amino acid residues identical.
RRID AB_2756735.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of rat brain membrane (lanes 1 and 5), mouse brain membrane (lanes 2 and 6), human acute lymphoblastic leukemia (MOLT-4) (lanes 3 and 7) and human colorectal adenocarcinoma (HT-29) cell lysates:
    Western blot analysis of rat brain membrane (lanes 1 and 5), mouse brain membrane (lanes 2 and 6), human acute lymphoblastic leukemia (MOLT-4) (lanes 3 and 7) and human colorectal adenocarcinoma (HT-29) cell lysates:
    1-4. Anti-LETM1 Antibody (#ANT-183), (1:1500).
    5-8. Anti-LETM1 Antibody, preincubated with LETM1 Blocking Peptide (#BLP-NT183).
References
  1. Nowikovsky, K. et al. (2012) J. Gen. Physiol. 139, 445.
Scientific background

LETM1 (leucine zipper–EF hand-containing transmembrane region), a protein with a key role in mitochondrial volume homeostasis through regulation of K+/H+ exchange (KHE), is involved in respiratory chain biogenesis and in the pathogenesis of seizures in the Wolf–Hirschhorn syndrome (WHS). LETM1 has been recently proposed to catalyze mitochondrial H+/Ca2+ exchange, which would imply a role in mitochondrial Ca2+ homeostasis as well.

LETM1 contains a hydrophobic N-terminal portion spanning the inner membrane and a large hydrophilic portion including the C-terminus located in the matrix. The primary amino acid sequence of the C-terminal part predicts at least two coiled-coil domains and two Ca2+-binding EF hand–like motifs. However, the EF hands differ from canonical EF hand motifs by several residues, and the α helices are not directly flanking the Ca2+-binding loop. The amino acid sequence of yeast LETM1 proteins lacks these putative Ca2+-binding EF hand motifs. The N-terminal region of the LETM1 protein superfamily displays a mitochondrial targeting sequence and a single transmembrane domain. The latter is highly conserved in all orthologues and is rich in proline residues.

In mitochondria, absence of LETM1, causes decompensation of electrophoretic K+, resulting in matrix swelling causing, in turn, increased mitophagy and/or cell death1.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Anti-LETM1 Antibody (#ANT-183) is a highly selective antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize LETM1 from human, mouse, and rat samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

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