Anti-LPAR2 (extracellular)-FITC Antibody

Lysophosphatidic acid receptor 2, LPA receptor 2, Lysophosphatidic acid receptor Edg-4
  • New
Cat #: ALR-032-F
Sizes: 50 µl
  • Lyophilized Powder
  • Antigen Incl.
  • FITC
  • QC Tested
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)NSGKELSLHWRPKD, corresponding to amino acid residues 16-29 of mouse LPA2 Receptor (Accession Q9JL06). Extracellular, N-terminus.
    Accession (Uniprot) Number Q9JL06
    Gene ID 53978
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat - identical; human - 13/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Label Fluorescein isothiocyanate (FITC)
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #ALR-032), and direct flow cytometry (labeled antibody).
    Applications: fc
    May also work in: ic*, lci*
    Direct flow cytometry
    Direct flow cytometry of live intact human THP-1 monocytic leukemia cells:
    ___ Cells.
    ___ Cells + rabbit IgG isotype control-FITC.
    ___ Cells + Anti-LPA2 Receptor (extracellular) Antibody-FITC (#ALR-032-F), (2.5 µg).
    Direct flow cytometry of live intact mouse J774 macrophage cells:
    ___ Cells.
    ___ Cells + rabbit IgG isotype control-FITC.
    ___ Cells + Anti-LPA2 Receptor (extracellular) Antibody-FITC (#ALR-032-F), (5 µg).
    References
    1. Yun, C.C. et al.  (2005) Am. J. Physiol. 289, C2.
    2. Feng, Y. et al. (2014) Exp. Ther. Med7, 423.
    3. Knowlden, S.  et al. (2013) Atlas of Genetics and Cytogenetics in Oncology and Haematology 17, 259.
    4. Contos, J.J. et al. (2002) Mol. Cell. Biol. 22, 6921.
    5. Fang, X. et al. (2000) Ann. N.Y. Acad. Sci. 905, 188.
    Scientific background

    Lysophosphatidic acid (LPA) is a lipid mediator that induces various cellular responses including proliferation, migration and inhibition of apoptosis in numerous types of cells via its specific G protein-coupled receptors. At least 5 LPA G-protein coupled receptors (GPCRs) have been identified so far. LPA mediates its effects predominantly by interacting with members of the endothelial differentiation gene (EDG) subfamily of GPCRs: LPA1/EDG2, LPA2/EDG4, and LPA3/EDG71. LPA2 couples to three distinct families of heterotrimeric GTP binding proteins, including Gαq, Gαi, and Gα12/132.

    LPA2 spans the plasma membrane seven times and contains three extracellular loops and three intracellular loops. LPA2 is unique from the other LPA receptors as it contains two distinct protein-protein interaction domains in the carboxyl-terminal tail (aa 296-351). In the proximal region, LPA2 contains a di-leucine motif and several putative palmitoylated cysteine residues. This region is responsible for associating with zinc-finger proteins3.

    LPA2 mRNA is expressed in a variety of tissues including human testis, leukocytes, prostate, spleen, thymus, pancreas, and bone marrow3.

    LPA2-null mice do not show any obvious phenotypic aberrations5. On the other hand, LPA2 expression is increased in ovarian and thyroid cancers and inflammation, suggesting that the main function of LPA2 could become evident under pathophysiological conditions6.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 11/06/2018

    Anti-LPAR2 (extracellular) Antibody (#ALR-032) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It recognizes an extracellular epitope and thus is ideal for detecting the receptor in living cells. It has been designed to recognize LPA2 Receptor from human, rat, and mouse samples.

    Anti-LPAR2 (extracellular)-FITC Antibody (#ALR-032-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

    For research purposes only, not for human use